比较 Sensititre YeastOne 抗真菌方法与 CLSI M27-A3 参考方法，以确定抗真菌药物对念珠菌属临床分离株的活性。
Comparison of the Sensititre YeastOne antifungal method with the CLSI M27-A3 reference method to determine the activity of antifungal agents against clinical isolates of Candidaspp.
机构信息
Department of Microbiology, Division of Mycology, Eskişehir City Hospital, Eskişehir, Turkey
Department of Microbiology, Division of Mycology Şişli Hamidiye Etfal Training and Research Hospital, İstanbul, Turkey
出版信息
Turk J Med Sci. 2020 Dec 17;50(8):2024-2031. doi: 10.3906/sag-1909-97.
BACKGROUND AND AIM
Infections caused by Candida species are significantly increasing today, and invasive Candida infections are generally associated with high mortality. Early diagnosis and identification of Candida spp. is important for the determination of antifungal agents that will be used for treatment. The aim of the present study was to provide a better regimen for Candida infections in the future.
MATERIALS AND METHODS
TheSensititre YeastOne (SYO) method was compared with The Clinical Laboratory Standards Institute (CLSI) reference broth microdilution (BMD) testing method. Endpoints of minimal inhibitory concentrations (MICs) were determined for both methods.
RESULTS
By using both methods, MIC values of micafungin, caspofungin, voriconazole, and fluconazole were lower than amphotericin B. The values obtained with the SYO method were in high categorical agreement for ecinocandins and amphotericin B. The results of voriconazole and fluconazole were in low categorical agreement. The categorical agreement between the SYO and the BMD results at 24 h was 82.1% for VORI and 98.4% for AMB. Values obtained with SYO method for all antifungal agents were in high essential agreement with the data of the CLSI reference BMD method. The essential agreement between the SYO and the BMD results at 24 h was 94.0% for MFG and 99.0% for AMB.
CONCLUSIONS
The SYO method was ready-to use, so it appeared to be easier and more efficient for Candida isolates.
背景与目的
如今，由念珠菌属引起的感染显著增加，侵袭性念珠菌感染通常与高死亡率相关。早期诊断和鉴定念珠菌属对于确定将用于治疗的抗真菌药物非常重要。本研究旨在为未来的念珠菌感染提供更好的治疗方案。
材料与方法
比较 Sensititre YeastOne（SYO）方法和临床和实验室标准协会（CLSI）参考肉汤微量稀释（BMD）检测方法。确定两种方法的最小抑菌浓度（MIC）终点。
结果
使用这两种方法，米卡芬净、卡泊芬净、伏立康唑和氟康唑的 MIC 值均低于两性霉素 B。SYO 法测定的棘白菌素和两性霉素 B 的 MIC 值具有较高的分类一致性。伏立康唑和氟康唑的结果具有较低的分类一致性。24 小时时 SYO 与 BMD 结果的分类一致性为 82.1%（VORI）和 98.4%（AMB）。SYO 法测定的所有抗真菌药物的 MIC 值与 CLSI 参考 BMD 方法的数据具有高度的临床一致性。24 小时时 SYO 与 BMD 结果的临床一致性为 94.0%（MFG）和 99.0%（AMB）。
结论
SYO 法即开即用，因此对于念珠菌分离株来说似乎更容易且更高效。
Zotero 插件