Department of Biomedical Engineering, Sogang University, Baekbeom-ro 35, Mapo-gu, Seoul 04107, Korea.
Department of Mechanical Engineering, Sogang University, Baekbeom-ro 35, Mapo-gu, Seoul 04107, Korea.
Int J Mol Sci. 2020 Jul 9;21(14):4858. doi: 10.3390/ijms21144858.
Heat stress negatively impacts plant growth and yield. The effects of carbon materials on plants in response to abiotic stress and antioxidant activity are poorly understood. In this study, we propose a new method for improving heat tolerance in cucumber ( L.) using a natural carbon material, shungite, which can be easily mixed into any soil. We analyzed the phenotype and physiological changes in cucumber plants maintained at 35 °C or 40 °C for 1 week. Our results show that shungite-treated cucumber plants had a healthier phenotype, exhibiting dark green leaves, compared to the plants in the control soil group. Furthermore, in the shungite-treated plants, the monodehydroascorbate content (a marker of oxidative damage) of the leaf was 34% lower than that in the control group. In addition, scavengers against reactive oxygen species, such as superoxide dismutase, catalase, and peroxidase were significantly upregulated. These results indicate that the successive pre-treatment of soil with a low-cost natural carbon material can improve the tolerance of cucumber plants to heat stress, as well as improve the corresponding antioxidant activity.
热应激会对植物的生长和产量产生负面影响。关于碳材料对植物应对非生物胁迫和抗氧化活性的影响，我们知之甚少。在这项研究中，我们提出了一种使用天然碳材料——陨墨烯来提高黄瓜（L.）耐热性的新方法，这种方法可以很容易地混入任何土壤中。我们分析了在 35°C 或 40°C 下维持一周的黄瓜植株的表型和生理变化。结果表明，与对照土壤组相比，陨墨烯处理的黄瓜植株具有更健康的表型，叶片呈深绿色。此外，在陨墨烯处理的植株中，单脱氢抗坏血酸（氧化损伤的标志物）的含量比对照组低 34%。此外，活性氧清除剂，如超氧化物歧化酶、过氧化氢酶和过氧化物酶，显著上调。这些结果表明，用低成本的天然碳材料对土壤进行连续预处理可以提高黄瓜植株对热应激的耐受性，并提高相应的抗氧化活性。
