Department of Applied Biosciences, Kyungpook National University, Daegu, 41566, Korea.
Department of Plant Medicals, Andong National University, Andong, Republic of Korea.
BMC Plant Biol. 2024 Jun 10;24(1):523. doi: 10.1186/s12870-024-05240-z.
Allelopathy is a biological process in which one organism releases biochemicals that affect the growth and development of other organisms. The current investigation sought to determine the allelopathic effect of Rumex acetosella on white clover (Trifolium repens) growth and development by using its shoot extract (lower IC value) as a foliar treatment. Here, different concentrations (25, 50, 100, and 200 g/L) of shoot extract from Rumex acetosella were used as treatments. With increasing concentrations of shoot extract, the plant growth parameters, chlorophyll and total protein content of Trifolium repens decreased. On the other hand, ROS, such as O and HO and antioxidant enzymes, including SOD, CAT, and POD, increased with increasing shoot extract concentration. A phytohormonal study indicated that increased treatment concentrations increased ABA and SA levels while JA levels were reduced. For the identification of allelochemicals, liquid‒liquid extraction, thin-layer chromatography, and open-column chromatography were conducted using R. acetosella shoot extracts, followed by a seed bioassay on the separated layer. A lower IC value was obtained through GC/MS analysis. gammaSitosterol was identified as the most abundant component. The shoot extract of Rumex acetosella has strong allelochemical properties that may significantly impede the growth and development of Trifolium repens. This approach could help to understand the competitive abilities of this weed species and in further research provide an alternate weed management strategy.
化感作用是一种生物过程，其中一种生物释放生化物质，影响其他生物的生长和发育。本研究旨在通过利用其茎叶提取物（较低的 IC 值）作为叶面处理，确定水酸模对白三叶草（Trifolium repens）生长和发育的化感作用。在这里，使用不同浓度（25、50、100 和 200 g/L）的水酸模茎叶提取物作为处理。随着茎叶提取物浓度的增加，白三叶草的植物生长参数、叶绿素和总蛋白含量降低。另一方面，随着茎叶提取物浓度的增加，ROS（如 O 和 HO）和抗氧化酶（如 SOD、CAT 和 POD）增加。植物激素研究表明，处理浓度的增加会增加 ABA 和 SA 水平，而 JA 水平会降低。为了鉴定化感物质，使用水酸模茎叶提取物进行液-液萃取、薄层层析和开放柱层析，然后对分离层进行种子生物测定。通过 GC/MS 分析获得较低的 IC 值。γ-谷甾醇被鉴定为最丰富的成分。水酸模茎叶提取物具有很强的化感特性，可能会严重阻碍白三叶草的生长和发育。这种方法可以帮助了解这种杂草的竞争能力，并在进一步的研究中提供一种替代杂草管理策略。
