CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Centre of Chemistry for Energy Materials, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
Anal Chem. 2020 Aug 4;92(15):10677-10685. doi: 10.1021/acs.analchem.0c01779. Epub 2020 Jul 24.
A stimuli-responsive chemiluminescent nanocomposite was developed based on bimetallic nanoclusters encapsulated in a reactive oxygen species (ROS)-responsive polymer for a highly sensitive determination of ROS (i.e., HO) in biological samples. Cobalt/gold bimetallic nanoclusters (GSH@Co-AuNCs) were synthesized using glutathione (GSH) as a reducing-cum-protecting reagent. The GSH-coated nanoclusters were covalently bound to -(4-aminobutyl)--ethylisoluminol (ABEI) to form the GSH@Co-AuNCs-ABEI, which was further encapsulated in polymeric ROS-sensitive boronic ester modified dextran (Oxi-Dex) nanospheres through hydrophobic interactions. The stimulation of HO, as a model ROS, led to a sustainable structural cleavage of the Co-AuNCs-ABEI@Oxi-Dex nanocomposites and release of internal GSH@Co-AuNCs-ABEI, accompanied by intense chemiluminescence (CL). On this basis, an enzyme-free and reagent-free CL sensor was developed for a highly sensitive and selective determination of HO with a detection limit of 35.8 pM in biological samples. It is of great application potential for the determination of ROS related to various diseases.
基于封装在活性氧（ROS）响应聚合物中的双金属纳米簇的刺激响应化学发光纳米复合材料被开发出来，用于在生物样品中高灵敏度地测定 ROS（即 HO）。使用谷胱甘肽（GSH）作为还原-保护试剂合成了钴/金双金属纳米簇（GSH@Co-AuNCs）。GSH 涂层的纳米簇通过共价键结合到 -（4-氨基丁基）-乙基异鲁米诺（ABEI）上，形成 GSH@Co-AuNCs-ABEI，然后通过疏水相互作用进一步封装在具有聚合 ROS 敏感硼酸酯修饰的葡聚糖（Oxi-Dex）纳米球中。HO 作为模型 ROS 的刺激导致 Co-AuNCs-ABEI@Oxi-Dex 纳米复合材料的可持续结构裂解和内部 GSH@Co-AuNCs-ABEI 的释放，伴随着强烈的化学发光（CL）。在此基础上，开发了一种无酶和无试剂的 CL 传感器，用于高度灵敏和选择性地测定生物样品中 HO，检测限为 35.8 pM。它在测定与各种疾病相关的 ROS 方面具有很大的应用潜力。
