Unidad Especializada Laboratorio Nacional de Investigación para la Inocuidad Alimentaria (LANIIA)-Unidad Nayarit, Centro Nayarita de Innovación y Transferencia de Tecnología A.C., Tepic, México.
Laboratorio de Inmunotoxicología, Universidad Autónoma de Nayarit. Secretaría de Investigación y Posgrado, Tepic, México.
Int J Environ Health Res. 2022 Apr;32(4):701-711. doi: 10.1080/09603123.2020.1791803. Epub 2020 Jul 14.
Temephos and spinosad are pesticides used for control of vector-borne diseases such as dengue, chikungunya and zika. However, the inadequate use of these substances has affected the health of non-target organisms. The aim of this study was to evaluate and compare, the effects of temephos and spinosad on leukocyte viability and death, using guppy fish ( as a model organism. Guppies were exposed to temephos (10 mg/L) and spinosad (0.5 mg/L) for 7, 14, and 21 days. Afterwards, they were placed in pesticide-free fish tanks (7, 35, and 70 days) for recovery. The results showed that exposure to temephos caused leukocyte death, even at 35 days of recovery. Contrarily, the exposure to spinosad did not cause leukocyte death. This research show, for the first time, that a single dose of temephos causes apoptosis up to 56 days post-exposition, indicating that this pesticide induces chronic effects on immune response cells.
双硫磷和多杀菌素是用于控制登革热、基孔肯雅热和寨卡等虫媒疾病的杀虫剂。然而，这些物质的使用不当已经影响到非目标生物的健康。本研究旨在评估和比较双硫磷和多杀菌素对丽鱼（作为模式生物）白细胞活力和死亡的影响。丽鱼暴露于双硫磷（10mg/L）和多杀菌素（0.5mg/L）中 7、14 和 21 天。之后，它们被放置在无杀虫剂的鱼缸中（7、35 和 70 天）进行恢复。结果表明，即使在恢复 35 天后，暴露于双硫磷也会导致白细胞死亡。相反，暴露于多杀菌素不会导致白细胞死亡。这项研究首次表明，单次双硫磷剂量会导致暴露后 56 天的细胞凋亡，表明这种杀虫剂会对免疫反应细胞产生慢性影响。
