Coles F B, Cartun R W, Pastuszak W T
Department of Pathology, Hartford Hospital, Connecticut.
Mod Pathol. 1988 Jul;1(4):274-8.
Utilizing the monoclonal antibodies L26 (a new antibody possessing immunoreactivity with B-lymphocytes in paraffin-embedded tissue), LN1, LN2, and Leu-M1, 44 cases of Hodgkin's disease (HD) were examined for the presence of immunoreactivity in Reed-Sternberg (R-S) cells by the avidin-biotin-peroxidase complex (ABC) technique. In 16 cases of lymphocyte-predominant Hodgkin's disease (LPHD), the L&H variants of R-S cells exhibited a different pattern of staining compared to R-S cells in other histologic types (total, 28 cases: 11, mixed cellularity; 8, nodular sclerosing; 6, lymphocyte depleted; 3, unclassified). L&H variants in LPHD were immunoreactive for L26 and LN1 in 15 and 14 cases, respectively, whereas R-S cells in the remaining types were negative or rarely positive (3, L26; 2, LN1). Leu-M1 was strongly positive in 27 of 28 cases of non-LPHD versus only 4 of 16 in LPHD. LN2 was reactive in virtually all cases (43 of 44). These findings suggest the possibility that the R-S cells of LPHD are derived from a different lineage than R-S cells in other histologic types of HD or that the latter have somehow lost the ability to express the antigens defined by L26 and LN1. Finally, based on immunologic and morphologic findings in this study, the similarities seen between the nodular and diffuse subtypes of LPHD are felt to favor a close relationship between the two subtypes.
利用单克隆抗体L26（一种对石蜡包埋组织中的B淋巴细胞具有免疫反应性的新抗体）、LN1、LN2和Leu-M1，采用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）技术检测了44例霍奇金病（HD）的里德-施特恩伯格（R-S）细胞中免疫反应性的存在情况。在16例淋巴细胞为主型霍奇金病（LPHD）中，R-S细胞的L&H变异型与其他组织学类型（共28例：11例混合细胞型、8例结节硬化型、6例淋巴细胞消减型、3例未分类）中的R-S细胞相比，呈现出不同的染色模式。LPHD中的L&H变异型分别在15例和14例中对L26和LN1呈免疫反应性，而其余类型中的R-S细胞为阴性或很少呈阳性（L26，3例；LN1，2例）。Leu-M1在28例非LPHD中有27例呈强阳性，而在LPHD的16例中只有4例呈强阳性。LN2在几乎所有病例（44例中的43例）中均有反应性。这些发现提示，LPHD的R-S细胞可能与其他组织学类型HD中的R-S细胞来源不同，或者后者以某种方式丧失了表达由L26和LN1所定义抗原的能力。最后，基于本研究中的免疫和形态学发现，LPHD结节型和弥漫型亚型之间的相似性被认为支持这两种亚型之间存在密切关系。
