Pinkus G S, Lones M, Shintaku I P, Said J W
Department of Pathology, Brigham and Women's Hospital, Boston, Massachussetts.
Mod Pathol. 1994 May;7(4):454-61.
A total of 186 specimens of Hodgkin's disease of various histologic types (127 nodular sclerosis, 39 mixed cellularity, 14 lymphocyte predominance, 3 lymphocyte depleted, and 3 unclassified) were evaluated for the presence of latent membrane protein (LMP) and Epstein-Barr virus nuclear antigen-2, two Epstein-Barr virus encoded gene products that appear to play important roles in cell transformation and oncogenesis. Immunoreactivity for LMP was observed in Reed-Sternberg cells and variants of 27/39 (69%) cases of mixed cellularity type, 18/127 (14%) of nodular sclerosis type, 2/3 cases of lymphocyte depleted type, and 1/3 cases of unclassified type. All cases of lymphocyte predominance Hodgkin's disease were nonreactive for LMP. In cases that were reactive for LMP, staining was restricted to Reed-Sternberg cells and variants. Other cells within the proliferation, e.g., lymphocytes, histiocytes, eosinophils, fibroblasts, etc., were nonreactive. The pattern of immunoreactivity for LMP was characterized by strong diffuse cytoplasmic staining, occasionally with membrane accentuation and/or paranuclear staining. Reactivity for LMP was demonstrated in cryostat sections and was also well preserved in paraffin sections of B5- or formalin-fixed tissues. Five of six specimens of Hodgkin's disease (4 mixed cellularity and 2 nodular sclerosis type) that occurred in HIV-positive patients exhibited immunoreactivity for LMP in Reed-Sternberg cells and variants. Cryostat section studies for Epstein-Barr virus nuclear antigen-2 using monoclonal antibody PE-2 failed to reveal staining for 43 cases (26 nodular sclerosis, 12 mixed cellularity, and 5 lymphocyte predominance) after a 2-h incubation with primary antibody.(ABSTRACT TRUNCATED AT 250 WORDS)
对总共186例不同组织学类型的霍奇金病标本（127例结节硬化型、39例混合细胞型、14例淋巴细胞为主型、3例淋巴细胞消减型和3例未分类型）进行评估，以检测潜伏膜蛋白（LMP）和爱泼斯坦-巴尔病毒核抗原2的存在，这两种爱泼斯坦-巴尔病毒编码的基因产物似乎在细胞转化和肿瘤发生中起重要作用。在39例混合细胞型中的27例（69%）、127例结节硬化型中的18例（14%）、3例淋巴细胞消减型中的2例以及3例未分类型中的1例的里德-斯腾伯格细胞及其变异细胞中观察到LMP免疫反应性。所有淋巴细胞为主型霍奇金病病例对LMP均无反应。在对LMP有反应的病例中，染色仅限于里德-斯腾伯格细胞及其变异细胞。增殖区内的其他细胞，如淋巴细胞、组织细胞、嗜酸性粒细胞、成纤维细胞等均无反应。LMP免疫反应性模式的特征为强烈的弥漫性细胞质染色，偶尔伴有膜增强和/或核旁染色。在冰冻切片中证实了对LMP的反应性，并且在B5或福尔马林固定组织的石蜡切片中也保存良好。在HIV阳性患者中发生的6例霍奇金病标本（4例混合细胞型和2例结节硬化型）中有5例在里德-斯腾伯格细胞及其变异细胞中表现出对LMP的免疫反应性。使用单克隆抗体PE-2对爱泼斯坦-巴尔病毒核抗原2进行冰冻切片研究，在与一抗孵育2小时后，43例（26例结节硬化型、12例混合细胞型和5例淋巴细胞为主型）未显示染色。（摘要截于250字）
