College of Food and Pharmacy, Zhejiang Ocean University, 1 South Haida Road, Zhoushan 316000, People's Republic of China.
Qingdao Academy of Agricultural Science, Qingdao 216000, People's Republic of China.
Int J Biol Macromol. 2020 Dec 1;164:87-94. doi: 10.1016/j.ijbiomac.2020.07.080. Epub 2020 Jul 11.
A sulfated fucan was extracted, purified, and characterized from Acaudina leucoprocta (a low value sea cucumber) to better understand and utilize this species. The structure of the sulfated fucan was elucidated using chemical and modern spectroscopic analyses including HPGPC, IR, AFM, GC-MS, and NMR, and its bioactivity was investigated. Our results showed that the sulfated fucan was mainly composed of → 3)-α-L-Fucp-(1→ linkage, and that the sulfate groups were substituted at the O-2 and/or O-4 positions of the fucose ring. In detail, the sulfated fucan consisted of Fuc0S (40%), Fuc2S4S (24%), Fuc2S (24%), and Fuc4S (12%). On average, there were seven sulfate groups on every eight fucose residues. Assay for anticoagulant activity indicated that the sulfated fucan displayed intrinsic anticoagulant activity and specific anti-thrombin activity through heparin cofactor II. Our results showed that this bioactive sulfated fucan could enable the high-value utilization of this low-value sea cucumber.
从糙海参（一种低值海参）中提取、纯化并表征了一种硫酸化岩藻聚糖，以更好地了解和利用该物种。使用化学和现代光谱分析（包括 HPGPC、IR、AFM、GC-MS 和 NMR）阐明了硫酸化岩藻聚糖的结构，并研究了其生物活性。结果表明，硫酸化岩藻聚糖主要由→3）-α-L-岩藻糖基-（1→键组成，硫酸基团取代在岩藻糖环的 O-2 和/或 O-4 位置。详细地说，硫酸化岩藻聚糖由 Fuc0S（40%）、Fuc2S4S（24%）、Fuc2S（24%）和 Fuc4S（12%）组成。平均每 8 个岩藻糖残基就有 7 个硫酸基。抗凝血活性测定表明，硫酸化岩藻聚糖通过肝素辅因子 II 显示出内在的抗凝血活性和特定的抗凝血酶活性。我们的结果表明，这种具有生物活性的硫酸化岩藻聚糖可以实现这种低值海参的高值利用。
