University of Tunis El Manar, Faculty of Medicine of Tunis, LR99ES11, Laboratory of Biochemistry, Rabta Hospital, Tunis, Tunisia; University of Tunis El Manar, Faculty of Sciences of Tunis, Tunisia.
University of Tunis El Manar, Faculty of Medicine of Tunis, LR99ES11, Laboratory of Biochemistry, Rabta Hospital, Tunis, Tunisia.
Cytokine. 2020 Oct;134:155195. doi: 10.1016/j.cyto.2020.155195. Epub 2020 Jul 11.
The pathogenesis of psoriasis is characterized by a disruption of extracellular matrix (ECM) in which matrix metalloproteinases (MMPs) participate actively. We aimed to determine MMP-7 level and its association with the inflammatory response in order to determine its usefulness as a biomarker for psoriasis prediction. We also aimed to determine its distribution in uninvolved and involved psoriatic skin to evaluate the probable role of MMP-7 in psoriasis pathogenesis.
We recruited 108 psoriatic patients and 133 healthy controls. MMP-7, tissue inhibitors of metalloproteinases (TIMPs) and interleukin-6 (IL-6) levels were measured by Enzyme-Linked Immunosorbent Assay (ELISA) assay. MMP-7 expression was detected by Immunohistochemistry (IHC) study.
ECM turnover and inflammatory biomarker levels were significantly higher in psoriatic patients. MMP-7 revealed to be independently associated to psoriasis even after adjustment for different models. The area under the curve (AUC) of MMP-7 and inflammation Z-score were similar. MMP-7 was positively correlated with IL-6 and inflammation Z-score. Psoriasis severity (PASI) was correlated significantly with IL-6 (p = 0.007). The MMP-7 expression was detected in the epidermis of involved and uninvolved psoriatic skin. In involved skin, MMP-7 was expressed by basal and mostly suprabasal keratinocytes. In uninvolved skin, expression of MMP-7 was restricted to basal keratinocytes.
MMP-7 is independently associated to psoriasis disease and to inflammatory response which make it a potential biomarker for this dermatosis.
银屑病的发病机制表现为细胞外基质（ECM）的破坏，其中基质金属蛋白酶（MMPs）积极参与。我们旨在确定 MMP-7 水平及其与炎症反应的关联，以确定其作为预测银屑病的生物标志物的有用性。我们还旨在确定其在未受累和受累银屑病皮肤中的分布，以评估 MMP-7 在银屑病发病机制中的可能作用。
我们招募了 108 名银屑病患者和 133 名健康对照者。通过酶联免疫吸附试验（ELISA）测定 MMP-7、金属蛋白酶组织抑制剂（TIMPs）和白细胞介素 6（IL-6）水平。通过免疫组织化学（IHC）研究检测 MMP-7 表达。
银屑病患者 ECM 周转率和炎症生物标志物水平显著升高。MMP-7 被证明即使在调整不同模型后也与银屑病独立相关。MMP-7 和炎症 Z 分数的曲线下面积（AUC）相似。MMP-7 与 IL-6 和炎症 Z 分数呈正相关。银屑病严重程度（PASI）与 IL-6 显著相关（p=0.007）。MMP-7 在受累和未受累银屑病皮肤的表皮中均有表达。在受累皮肤中，MMP-7 由基底和大多数基底上层角质形成细胞表达。在未受累皮肤中，MMP-7 的表达仅限于基底角质形成细胞。
MMP-7 与银屑病疾病和炎症反应独立相关，使其成为这种皮肤病的潜在生物标志物。
