Lyons D J, Gao L, Mitchell E B, Mitchell D N
Division of Immunological Medicine, Clinical Research Centre, Harrow, London.
Thorax. 1988 Dec;43(12):992-7. doi: 10.1136/thx.43.12.992.
Interleukin-2 has been reported to enhance the immune response in diseases characterised by defective cell mediated immunity. The effect of exogenous recombinant interleukin-2 was studied on the proliferative and cytotoxic responses of peripheral blood mononuclear cells from 39 patients with sarcoidosis and 14 healthy control subjects. The proliferative response to purified protein derivative was smaller in patients than in control subjects (p less than 0.001) whereas the response to 80 U interleukin-2 alone and to purified protein derivative and interleukin-2 did not differ significantly between the two groups. In addition, in eight patients but no control subjects tritiated thymidine incorporation induced by the combination of purified protein derivative and interleukin-2 was more than twice the sum of that induced by purified protein derivative and interleukin separately. Cytotoxic activity occurring spontaneously and induced by purified protein derivative and interleukin-2 in blood mononuclear cells was significantly less for patients with sarcoidosis than for control subjects (p less than 0.05 spontaneous, less than 0.001 purified protein derivative induced, less than 0.02 interleukin induced). Synergism between antigen and interleukin did not occur with respect to the cytotoxic response in either patients or controls. Defective interleukin-2 production may contribute to, but does not entirely explain, the functional abnormalities of peripheral blood lymphocytes from patients with sarcoidosis.
据报道，白细胞介素 -2 可增强以细胞介导免疫缺陷为特征的疾病中的免疫反应。研究了外源性重组白细胞介素 -2 对 39 例结节病患者和 14 名健康对照者外周血单个核细胞增殖和细胞毒性反应的影响。患者对纯化蛋白衍生物的增殖反应小于对照者（p<0.001），而两组对单独 80U 白细胞介素 -2 以及对纯化蛋白衍生物和白细胞介素 -2 的反应无显著差异。此外，在 8 例患者中，纯化蛋白衍生物和白细胞介素 -2 联合诱导的氚标记胸腺嘧啶核苷掺入量超过纯化蛋白衍生物和白细胞介素分别诱导量之和的两倍，而对照者中无此现象。结节病患者血单个核细胞中自发出现的以及由纯化蛋白衍生物和白细胞介素 -2 诱导的细胞毒性活性明显低于对照者（自发的 p<0.05，纯化蛋白衍生物诱导的 p<0.001，白细胞介素诱导的 p<0.02）。在患者或对照者中，抗原和白细胞介素之间在细胞毒性反应方面均未出现协同作用。白细胞介素 -2 产生缺陷可能促成但不能完全解释结节病患者外周血淋巴细胞的功能异常。
