Division of Infectious Diseases, Brigham and Women's Hospital, United States; Department of Microbiology, Harvard Medical School, United States; Howard Hughes Medical Institute, United States.
Division of Infectious Diseases, Brigham and Women's Hospital, United States; Department of Microbiology, Harvard Medical School, United States; Howard Hughes Medical Institute, United States.
Curr Opin Microbiol. 2020 Oct;57:41-48. doi: 10.1016/j.mib.2020.06.005. Epub 2020 Jul 11.
Transfer RNAs (tRNAs) are non-coding RNAs essential for protein synthesis. tRNAs are heavily decorated with a variety of post-transcriptional modifications (tRNA modifications). Recent methodological advances provide new tools for rapid profiling of tRNA modifications and have led to discoveries of novel modifications and their regulation. Here, we provide an overview of the techniques for investigating tRNA modifications and of the expanding knowledge of their chemistry and regulation.
转移 RNA(tRNA)是蛋白质合成所必需的非编码 RNA。tRNA 经过多种转录后修饰(tRNA 修饰)的大量修饰。最近的方法学进展为 tRNA 修饰的快速分析提供了新的工具,并导致了新修饰及其调控的发现。在这里,我们提供了研究 tRNA 修饰的技术概述,以及对其化学和调控的不断扩大的认识。