Muñoz Julián E, Ramirez Laura M, Dias Lucas Dos Santos, Rivas Laura A, Ramos Lívia S, Santos André L S, Taborda Carlos P, Parra-Giraldo Claudia M
MICROS Group, Medicine Traslacional Institute, School of Medicine and Health Sciences, Universidad del Rosario, Bogotá, D.C. 111221, Colombia.
Unidad de Proteómica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C. 110231, Colombia.
J Fungi (Basel). 2020 Jul 10;6(3):104. doi: 10.3390/jof6030104.
and complex ( var. and ) are phylogenetically related species that share some physiological features and habits. In the present study, we compared the virulence of these yeast species using two different experimental models: (i) larvae to evaluate the survival rate, fungal burden, histopathology and phagocytosis index and (ii) BALB/c mice to evaluate the survival. In addition, the fungal capacity to form biofilm over an inert surface was analyzed. Our results showed that in both experimental models, the animal survival rate was lower when infected with strains than the species complex. The hemocytes of showed a significantly reduced ability to phagocytize the most virulent strains forming the species complex. Interestingly, for , it was impossible to measure the phagocytosis index due to a general lysis of the hemocytes. Moreover, it was observed a greater capability of biofilm formation by compared to species complex. In conclusion, we observed that and complex have different levels of pathogenicity in the experimental models employed in the present study.
和复合体（变种和）是在系统发育上相关的物种，它们具有一些共同的生理特征和习性。在本研究中，我们使用两种不同的实验模型比较了这些酵母物种的毒力：（i）幼虫，以评估存活率、真菌负荷、组织病理学和吞噬指数；（ii）BALB/c小鼠，以评估存活率。此外，还分析了真菌在惰性表面形成生物膜的能力。我们的结果表明，在两种实验模型中，感染菌株时动物的存活率均低于物种复合体。的血细胞对构成物种复合体的最具毒力菌株的吞噬能力显著降低。有趣的是，对于，由于血细胞普遍裂解，无法测量吞噬指数。此外，与物种复合体相比，观察到形成生物膜的能力更强。总之，我们观察到在本研究采用的实验模型中，和物种复合体具有不同程度的致病性。
