Xiao Wei, Zhou Hao, Huang Jian, Xin Caiyan, Zhang Jinping, Wen Huan, Song Zhangyong
Public Center of Experimental Technology, School of Basic Medical Sciences, Southwest Medical University, Luzhou, China.
Department of Medical Laboratory, Xuyong County People's Hospital, Luzhou, China.
Appl Environ Microbiol. 2025 Apr 23;91(4):e0240624. doi: 10.1128/aem.02406-24. Epub 2025 Mar 26.
and the emerging fungal pathogens comprising the complex ( and ) are phylogenetically closely related. Notably, each can cause serious nosocomial infection and acquire multidrug resistance. We isolated various strains of and complex from patient specimens. The results of growth curve analysis and the spot assay showed that was the most tolerant to high temperatures, while differences were found among the five strains of the complex. We selected a representative strain from each of the three types of fungi that exhibit differences in heat resistance for further research. In addition, three strains were resistant to fluconazole, whereas sensitivity to common antifungal agents differed, as determined by the micro liquid-based dilution method. Moreover, was more virulent in than members of the complex. In addition, there were notable differences in biofilm formation, hydrophobicity, adhesion, and enzyme activity among the three strains. The results of transcriptomics, real-time quantitative PCR, and biochemical analyses showed that was most tolerant to heat stress due to the expression of genes in regulating pyruvate consumption and the accumulation of intracellular pyruvate. These results provide valuable references for further studies of the biological characteristics, pathogenesis, and treatment of infection.
and the complex are multidrug-resistant fungi that have emerged in recent years, posing a significant threat to human health. The biological characteristics of two strains of the complex and one strain of isolated from clinical patient samples were analyzed. Our primary focus was to compare the heat resistance between and the complex, with a particular emphasis on understanding the differences in the heat resistance mechanisms. The main distinction between environmental and pathogenic fungi is that the latter can survive at human body temperature. Despite their close phylogenetic relationship, the complex and exhibit significant differences in heat resistance. Studying these heat resistance mechanisms may aid in our understanding of the evolutionary process of environmental fungi transforming into pathogenic fungi.
构成该复合体（和）的新兴真菌病原体在系统发育上密切相关。值得注意的是，每种都可引起严重的医院感染并获得多重耐药性。我们从患者标本中分离出了各种菌株的和复合体。生长曲线分析和斑点试验结果表明，对高温耐受性最强，而复合体的五个菌株之间存在差异。我们从三种耐热性不同的真菌类型中各选了一个代表性菌株进行进一步研究。此外，通过微量液体稀释法测定，有三种菌株对氟康唑耐药，而对常见抗真菌剂的敏感性不同。此外，在中比复合体的成员更具毒性。此外，三种菌株在生物膜形成、疏水性、粘附性和酶活性方面存在显著差异。转录组学、实时定量PCR和生化分析结果表明，由于调节丙酮酸消耗的基因表达和细胞内丙酮酸的积累，对热应激耐受性最强。这些结果为进一步研究感染的生物学特性、发病机制和治疗提供了有价值的参考。
和复合体是近年来出现的多重耐药真菌，对人类健康构成重大威胁。分析了从临床患者样本中分离出的复合体的两种菌株和一种菌株的生物学特性。我们主要关注的是比较和复合体之间的耐热性，特别强调了解耐热机制的差异。环境真菌和致病真菌的主要区别在于后者能在人体温度下存活。尽管它们在系统发育上关系密切，但复合体和在耐热性方面存在显著差异。研究这些耐热机制可能有助于我们理解环境真菌向致病真菌转变的进化过程。
