• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

粒细胞集落刺激因子（GCSF）通过上调背根神经节中早期 microRNA-122 的表达，抑制单核细胞趋化蛋白-1（MCP-1）的表达，从而减轻神经病理性疼痛。

Granulocyte Colony Stimulating Factor (GCSF) Can Attenuate Neuropathic Pain by Suppressing Monocyte Chemoattractant Protein-1 (MCP-1) Expression, through Upregulating the Early MicroRNA-122 Expression in the Dorsal Root Ganglia.

机构信息

Department of Life Science, National Taiwan Normal University, Taipei 11677, Taiwan.

Department of Neurology, Chang Gung Memorial Hospital-Linkou Medical Center and Chang Gung University College of Medicine, Taipei 33305, Taiwan.

出版信息

Cells. 2020 Jul 11;9(7):1669. doi: 10.3390/cells9071669.

DOI:10.3390/cells9071669
PMID:32664488
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7408430/
Abstract

Our previous animal studies and several human clinical trials have shown that granulocyte-colony stimulating factor (GCSF) can attenuate neuropathic pain through various mechanisms. GCSF itself is also a multipotent cytokine that can modulate microribonucleic acid (microRNA) expression profiles in vitro. In this study, we used the NanoString nCounter analysis system to screen the expression of different rodent microRNAs at early stage after nerve injury and studied the expression of related cytokines/chemokines in the dorsal root ganglia (DRGs) of rats that underwent chronic constriction injury (CCI) to explore the underlying mechanisms of the analgesic effects of GCSF. We found that microRNA-122 expression was downregulated by CCI; in contrast, GCSF treatment significantly upregulated microRNA-122 expression in the DRGs of CCI rats on the 1st day after nerve injury. We further studied the expression of different cytokines/chemokines (IL-1β, IL-6, and monocyte chemoattractant protein-1 (MCP-1)) that were modulated by microRNA-122. MCP-1 has been reported to participate in neuropathic pain development, and its expression on the DRGs of vehicle-treated CCI rats was significantly higher than that on the DRGs of sham-operated rats; in contrast, GCSF-treated rats exhibited significantly lower MCP-1 expression in the DRG than vehicle-treated rats on the 7th day after nerve injury. An early GCSF treatment can suppress MCP-1 expressions, through upregulating microRNA-122 expressions in the DRGs of CCI rats at an earlier stage, thus indirectly attenuating neuropathic pain development.

摘要

我们之前的动物研究和几项人类临床试验表明，粒细胞集落刺激因子（GCSF）可以通过多种机制减轻神经性疼痛。GCSF 本身也是一种多能细胞因子，能够在体外调节微小核糖核酸（microRNA）表达谱。在这项研究中，我们使用 NanoString nCounter 分析系统筛选了神经损伤后早期不同啮齿类动物 microRNA 的表达情况，并研究了慢性缩窄性损伤（CCI）大鼠背根神经节（DRG）中相关细胞因子/趋化因子的表达情况，以探讨 GCSF 镇痛作用的潜在机制。我们发现，CCI 可下调 microRNA-122 的表达；相反，CCI 大鼠神经损伤后第 1 天，GCSF 治疗可显著上调 DRG 中 microRNA-122 的表达。我们进一步研究了受 microRNA-122 调节的不同细胞因子/趋化因子（IL-1β、IL-6 和单核细胞趋化蛋白-1（MCP-1））的表达情况。MCP-1 已被报道参与神经性疼痛的发展，其在 vehicle 处理的 CCI 大鼠 DRG 中的表达明显高于 sham 手术大鼠；相反，GCSF 处理的大鼠在神经损伤后第 7 天，DRG 中 MCP-1 的表达明显低于 vehicle 处理的大鼠。早期 GCSF 治疗可以通过在 CCI 大鼠的 DRG 中更早地上调 microRNA-122 的表达，从而抑制 MCP-1 的表达，从而间接减轻神经性疼痛的发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/ee844bcfe169/cells-09-01669-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/447eaa31be92/cells-09-01669-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/5bf51d5860b3/cells-09-01669-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/1993657f4972/cells-09-01669-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/e5812f723349/cells-09-01669-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/6f9d06431d48/cells-09-01669-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/30ba52569a19/cells-09-01669-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/0ec250f25f06/cells-09-01669-g007a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/ee844bcfe169/cells-09-01669-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/447eaa31be92/cells-09-01669-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/5bf51d5860b3/cells-09-01669-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/1993657f4972/cells-09-01669-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/e5812f723349/cells-09-01669-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/6f9d06431d48/cells-09-01669-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/30ba52569a19/cells-09-01669-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/0ec250f25f06/cells-09-01669-g007a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3d/7408430/ee844bcfe169/cells-09-01669-g008.jpg

相似文献

1
Granulocyte Colony Stimulating Factor (GCSF) Can Attenuate Neuropathic Pain by Suppressing Monocyte Chemoattractant Protein-1 (MCP-1) Expression, through Upregulating the Early MicroRNA-122 Expression in the Dorsal Root Ganglia.粒细胞集落刺激因子（GCSF）通过上调背根神经节中早期 microRNA-122 的表达，抑制单核细胞趋化蛋白-1（MCP-1）的表达，从而减轻神经病理性疼痛。
Cells. 2020 Jul 11;9(7):1669. doi: 10.3390/cells9071669.
2
Early systemic granulocyte-colony stimulating factor treatment attenuates neuropathic pain after peripheral nerve injury.早期全身粒细胞集落刺激因子治疗可减轻周围神经损伤后的神经病理性疼痛。
PLoS One. 2012;7(8):e43680. doi: 10.1371/journal.pone.0043680. Epub 2012 Aug 24.
3
An early granulocyte colony-stimulating factor treatment attenuates neuropathic pain through activation of mu opioid receptors on the injured nerve.早期粒细胞集落刺激因子治疗通过激活损伤神经上的μ阿片受体减轻神经病理性疼痛。
Sci Rep. 2016 May 16;6:25490. doi: 10.1038/srep25490.
4
Activating Sirt1 by resveratrol suppresses Nav1.7 expression in DRG through miR-182 and alleviates neuropathic pain in rats.白藜芦醇通过 miR-182 激活 Sirt1 抑制 DRG 中 Nav1.7 的表达，从而减轻大鼠的神经性疼痛。
Channels (Austin). 2020 Dec;14(1):69-78. doi: 10.1080/19336950.2020.1732003.
5
MicroRNA-547-5p-mediated interleukin-33/suppressor of tumorigenicity 2 signaling underlies the genesis and maintenance of neuropathic pain and is targeted by the therapy with bone marrow stromal cells.微小 RNA-547-5p 介导的白细胞介素-33/肿瘤抑制因子 2 信号通路是神经病理性疼痛发生和维持的基础，骨髓基质细胞治疗可靶向该信号通路。
Mol Pain. 2020 Jan-Dec;16:1744806920931737. doi: 10.1177/1744806920931737.
6
Expression of monocyte chemoattractant protein-1 in rat dorsal root ganglia and spinal cord in experimental models of neuropathic pain.单核细胞趋化蛋白-1在神经性疼痛实验模型大鼠背根神经节和脊髓中的表达
Brain Res. 2009 Jan 28;1251:103-11. doi: 10.1016/j.brainres.2008.11.046. Epub 2008 Nov 27.
7
Expression changes of microRNA-1 and its targets Connexin 43 and brain-derived neurotrophic factor in the peripheral nervous system of chronic neuropathic rats.慢性神经性大鼠外周神经系统中微小RNA-1及其靶标连接蛋白43和脑源性神经营养因子的表达变化
Mol Pain. 2015 Jun 26;11:39. doi: 10.1186/s12990-015-0045-y.
8
Chronic constriction injury-induced microRNA-146a-5p alleviates neuropathic pain through suppression of IRAK1/TRAF6 signaling pathway.慢性缩窄性损伤诱导的 microRNA-146a-5p 通过抑制 IRAK1/TRAF6 信号通路缓解神经病理性疼痛。
J Neuroinflammation. 2018 Jun 9;15(1):179. doi: 10.1186/s12974-018-1215-4.
9
Effects of intermedin on dorsal root ganglia in the transmission of neuropathic pain in chronic constriction injury rats.肾上腺髓质素对慢性压迫性损伤大鼠背根神经节在神经性疼痛传递中的作用。
Clin Exp Pharmacol Physiol. 2015 Jul;42(7):780-7. doi: 10.1111/1440-1681.12416.
10
The Inhibition by Guanfu Base A of Neuropathic Pain Mediated by P2Y Receptor in Dorsal Root Ganglia.胍基福林 A 通过背根神经节 P2Y 受体对神经病理性疼痛的抑制作用。
ACS Chem Neurosci. 2019 Mar 20;10(3):1318-1325. doi: 10.1021/acschemneuro.8b00399. Epub 2018 Dec 10.

引用本文的文献

1
Molecular Adjuvant Potential of and in Starry Flounder Challenged with .在感染迟缓爱德华氏菌的星斑川鲽中[具体物质]的分子佐剂潜力
Animals (Basel). 2025 Jun 23;15(13):1848. doi: 10.3390/ani15131848.
2
Associations Between Circulating Inflammatory Cytokines and Neuropathic Pain: A Two-Sample Mendelian Randomization Study.循环炎症细胞因子与神经性疼痛之间的关联：一项两样本孟德尔随机化研究。
J Pain Res. 2025 Mar 24;18:1525-1544. doi: 10.2147/JPR.S495896. eCollection 2025.
3
Understanding painful versus non-painful dental pain in female and male patients: A transcriptomic analysis of human biopsies.

本文引用的文献

1
MiR-34a is differentially expressed in dorsal root ganglia in a rat model of chronic neuropathic pain.miR-34a 在慢性神经病理性疼痛大鼠模型的背根神经节中差异表达。
Neurosci Lett. 2019 Aug 24;708:134365. doi: 10.1016/j.neulet.2019.134365. Epub 2019 Jul 3.
2
Network and pathway-based analysis of microRNA role in neuropathic pain in rat models.基于网络和通路的 miRNA 在大鼠神经病理性疼痛模型中的作用分析。
J Cell Mol Med. 2019 Jul;23(7):4534-4544. doi: 10.1111/jcmm.14357. Epub 2019 May 8.
3
MicroRNA-182 Alleviates Neuropathic Pain by Regulating Nav1.7 Following Spared Nerve Injury in Rats.
理解女性和男性患者的疼痛性与非疼痛性牙科疼痛：人类活检的转录组分析。
PLoS One. 2023 Sep 21;18(9):e0291724. doi: 10.1371/journal.pone.0291724. eCollection 2023.
4
The Yin/Yang Balance of Communication between Sensory Neurons and Macrophages in Traumatic Peripheral Neuropathic Pain.创伤性周围神经病理性疼痛中感觉神经元与巨噬细胞之间通信的阴阳平衡
Int J Mol Sci. 2022 Oct 16;23(20):12389. doi: 10.3390/ijms232012389.
5
Preventive Intrathecal Injection of Bupivacaine Alleviated Microglia Activation and Neuropathic Pain in a Rat Model of Chronic Constriction Injury.布比卡因鞘内预防性注射减轻慢性缩窄性损伤大鼠模型中小胶质细胞激活和神经病理性疼痛。
Int J Mol Sci. 2022 Jun 28;23(13):7197. doi: 10.3390/ijms23137197.
6
Endogenous Expression of G-CSF in Rat Dorsal Root Ganglion Neurons after Nerve Injury.神经损伤后大鼠背根神经节神经元中G-CSF的内源性表达
Brain Sci. 2021 Jul 20;11(7):956. doi: 10.3390/brainsci11070956.
7
Circulating microRNA alternations in primary hyperuricemia and gout.原发性高尿酸血症和痛风患者循环 microRNA 的变化。
Arthritis Res Ther. 2021 Jul 10;23(1):186. doi: 10.1186/s13075-021-02569-w.
8
Interactions between Autophagy, Proinflammatory Cytokines, and Apoptosis in Neuropathic Pain: Granulocyte Colony Stimulating Factor as a Multipotent Therapy in Rats with Chronic Constriction Injury.自噬、促炎细胞因子与细胞凋亡在神经性疼痛中的相互作用：粒细胞集落刺激因子作为慢性缩窄性损伤大鼠的多效性治疗手段
Biomedicines. 2021 May 12;9(5):542. doi: 10.3390/biomedicines9050542.
微小 RNA-182 通过调控 spared 神经损伤大鼠的 Nav1.7 缓解神经病理性疼痛。
Sci Rep. 2018 Nov 13;8(1):16750. doi: 10.1038/s41598-018-34755-3.
4
Chronic constriction injury-induced microRNA-146a-5p alleviates neuropathic pain through suppression of IRAK1/TRAF6 signaling pathway.慢性缩窄性损伤诱导的 microRNA-146a-5p 通过抑制 IRAK1/TRAF6 信号通路缓解神经病理性疼痛。
J Neuroinflammation. 2018 Jun 9;15(1):179. doi: 10.1186/s12974-018-1215-4.
5
MiR-183-5p Alleviates Chronic Constriction Injury-Induced Neuropathic Pain Through Inhibition of TREK-1.miR-183-5p 通过抑制 TREK-1 缓解慢性缩窄性损伤诱导的神经性疼痛。
Neurochem Res. 2018 Jun;43(6):1143-1149. doi: 10.1007/s11064-018-2529-4. Epub 2018 May 7.
6
MicroRNA cluster miR-17-92 regulates multiple functionally related voltage-gated potassium channels in chronic neuropathic pain.miR-17-92 微RNA 簇调控慢性神经性疼痛中多个功能相关的电压门控钾通道。
Nat Commun. 2017 Jul 5;8:16079. doi: 10.1038/ncomms16079.
7
MicroRNA and chronic pain: From mechanisms to therapeutic potential.微小 RNA 与慢性疼痛：从机制到治疗潜力。
Pharmacol Ther. 2017 Dec;180:1-15. doi: 10.1016/j.pharmthera.2017.06.001. Epub 2017 Jun 1.
8
MicroRNA-122 can be measured in capillary blood which facilitates point-of-care testing for drug-induced liver injury.微 RNA-122 可在毛细血管血中测量，这有助于实现即时检测药物性肝损伤。
Br J Clin Pharmacol. 2017 Sep;83(9):2027-2033. doi: 10.1111/bcp.13282. Epub 2017 Apr 5.
9
Overexpression of miR-206 ameliorates chronic constriction injury-induced neuropathic pain in rats via the MEK/ERK pathway by targeting brain-derived neurotrophic factor.miR-206的过表达通过靶向脑源性神经营养因子，经由MEK/ERK途径改善大鼠慢性压迫性损伤诱导的神经性疼痛。
Neurosci Lett. 2017 Apr 12;646:68-74. doi: 10.1016/j.neulet.2016.12.047. Epub 2017 Jan 24.
10
An early granulocyte colony-stimulating factor treatment attenuates neuropathic pain through activation of mu opioid receptors on the injured nerve.早期粒细胞集落刺激因子治疗通过激活损伤神经上的μ阿片受体减轻神经病理性疼痛。
Sci Rep. 2016 May 16;6:25490. doi: 10.1038/srep25490.