Bikiaris Nikolaos D, Michailidou Georgia, Lazaridou Maria, Christodoulou Evi, Gounari Eleni, Ofrydopoulou Anna, Lambropoulou Dimitra A, Vergkizi-Nikolakaki Souzan, Lykidou Smaro, Nikolaidis Nikolaos
Laboratory of Chemistry and Technology of Polymers and Dyes, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Laboratory of Environmental Pollution Control, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Polymers (Basel). 2020 Jul 12;12(7):1542. doi: 10.3390/polym12071542.
In the present study, a chitosan (CS) derivative with the 2-(Methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (SDAEM) zwitterionic monomer was prepared through chemical modification. The successful synthesis of CS-SDAEM was confirmed by Fourier-transform Infrared (FTIR) and Nuclear Magnetic Resonance (H-NMR) spectroscopies. Its crystallinity was studied by X-ray Diffraction (XRD), while in vitro cytotoxicity and cell viability assays established its biocompatibility. Filtered fresh pomegranate juice (PJ) was loaded in nanoparticles of neat CS and its derivative via ionic gelation method. Dynamic Light Scattering (DLS) revealed nanoparticles sizes varying between 426 nm and 4.5 μm, indicating a size-dependence on the polymer concentration used during encapsulation. High-performance liquid chromatography coupled with photodiode array and electrospray ionization mass spectrometry detection (LC-PDA-ESI/MS) revealed that PJ active compounds were successfully and in sufficient amounts encapsulated in the nanoparticles interior, whereas XRD indicated a crystalline structure alteration after nanoencapsulation. The resulted PJ-loaded nanoparticles were further utilized for the preparation of innovative O/W cosmetic emulsions. All produced emulsions exhibited good pH and viscosity stability for up to 90 days, while the sun protection factor (SPF) was enhanced due to the presence of the PJ. Enhanced antioxidant and antimicrobial properties due to the phenolic compounds of PJ were also observed.
在本研究中，通过化学改性制备了一种含有2-（甲基丙烯酰氧基）乙基]二甲基-（3-磺丙基）氢氧化铵（SDAEM）两性离子单体的壳聚糖（CS）衍生物。傅里叶变换红外光谱（FTIR）和核磁共振（H-NMR）光谱证实了CS-SDAEM的成功合成。通过X射线衍射（XRD）研究了其结晶度，而体外细胞毒性和细胞活力测定确定了其生物相容性。通过离子凝胶法将过滤后的新鲜石榴汁（PJ）负载到纯CS及其衍生物的纳米颗粒中。动态光散射（DLS）显示纳米颗粒尺寸在426nm至4.5μm之间变化，表明其尺寸取决于封装过程中使用的聚合物浓度。高效液相色谱结合光电二极管阵列和电喷雾电离质谱检测（LC-PDA-ESI/MS）表明，PJ活性化合物已成功且足量地封装在纳米颗粒内部，而XRD表明纳米封装后晶体结构发生了改变。所得的负载PJ的纳米颗粒进一步用于制备创新的O/W型化妆品乳液。所有制备的乳液在长达90天的时间内均表现出良好的pH值和粘度稳定性，同时由于PJ的存在，防晒系数（SPF）得到了提高。还观察到由于PJ中的酚类化合物而增强的抗氧化和抗菌性能。
