Department of Biotechnology, Proteomics and Mass Spectrometry Laboratory, University of Verona, Verona, Italy.
Department of Medicine, Section of Internal Medicine D, University of Verona, Verona, Italy.
Sci Rep. 2020 Jul 14;10(1):11572. doi: 10.1038/s41598-020-68483-4.
The topical application of lactic acid bacteria (LAB) is recognized as a useful approach to improve skin health. This work aims to characterize by a multidisciplinary approach, the wound healing, anti-inflammatory, anti-pathogens and proteomic effects of six LAB lysates, belonging to the genus Lactobacillus. Our results demonstrated that the lysates of tested LAB stimulated the proliferation of keratinocytes, and that L. plantarum SGL 07 and L. salivarius SGL 19 accelerated the re-epithelization by inducing keratinocyte migration. The bacterial lysates also reduced the secretion of specific pro-inflammatory mediators from keratinocytes. Furthermore, viable L. salivarius SGL 19 and L. fermentum SGL 10 had anti-pathogenic effects against S. aureus and S. pyogenes, while L. brevis SGL 12 and L. paracasei SGL 04 inhibited S. aureus and S. pyogenes, respectively. The tested lactobacilli lysates also induced specific proteome modulation of the exposed keratinocytes, involving dysregulation of proteins (such as interleukin enhancer-binding factor 2 and ATP-dependent RNA helicase) and pathways (such as cytokine, NF-kB, Hedgehog, and RUNX signaling) associated with their specific wound healing and anti-inflammatory effects. This study indicates the different potential of selected lactobacilli, suggesting that they may be successfully used in the future together with conventional therapies to bring relief from skin disorders.
乳酸菌（LAB）的局部应用被认为是改善皮肤健康的一种有效方法。本工作旨在通过多学科方法，对属于乳杆菌属的六种 LAB 裂解物的伤口愈合、抗炎、抗病原体和蛋白质组学效应进行表征。我们的结果表明，测试的 LAB 裂解物刺激角质细胞增殖，并且 L. plantarum SGL 07 和 L. salivarius SGL 19 通过诱导角质细胞迁移加速再上皮化。细菌裂解物还减少了角质细胞特定促炎介质的分泌。此外，活的 L. salivarius SGL 19 和 L. fermentum SGL 10 对金黄色葡萄球菌和化脓性链球菌具有抗病原体作用，而 L. brevis SGL 12 和 L. paracasei SGL 04 分别抑制金黄色葡萄球菌和化脓性链球菌。测试的乳杆菌裂解物也诱导暴露的角质细胞特定的蛋白质组学调节，涉及蛋白质（如白细胞介素增强结合因子 2 和 ATP 依赖性 RNA 解旋酶）和途径（如细胞因子、NF-kB、Hedgehog 和 RUNX 信号）的失调，这些途径与它们特定的伤口愈合和抗炎作用有关。本研究表明了所选乳杆菌的不同潜力，表明它们将来可能与常规疗法一起成功用于缓解皮肤疾病。
