Guangxi Medical University Cancer Hospital, Nanning, Guangxi, China.
School of Public Health, Guangxi Medical University, Nanning, Guangxi, China.
Sci Rep. 2020 Jul 14;10(1):11573. doi: 10.1038/s41598-020-68450-z.
miRNAs play an indispensable role in human carcinogenesis. Dysregulated miR-1180-3p has been observed in several types of cancer, including hepatocellular carcinoma (HCC). This study intends to correlate the expression level of miR-1180-3p with clinical features and overall survival in HCC patients. The expression and clinical significance of miR-1180-3p, selected from GEO and TCGA databases, were verified using an RT-qPCR method. The target genes of miR-1180-3p were obtained using 3 miRNA target gene prediction databases, and their functions were analyzed using the online tool WebGestalt. miR-1180-3p expression was significantly upregulated in 88 HCC tissues compared with non-tumor liver tissues (0.004 ± 0.009 vs. 0.002 ± 0.002, t = - 2.099, P = 0.038). Additionally, we found that the expression levels of miR-1180-3p were significantly correlated with tumor number (χ = 9.157, P = 0.006) and MVI (χ = 11.354, P = 0.003). Based on Kaplan-Meier analysis, patients with high miR-1180 expression had a shorter overall survival than those with low miR-1180-3p expression (P = 0.002). Furthermore, multivariate Cox analyses indicated that miR-1180-3p expression was an independent prognostic factor for overall survival (HR = 13.36, 95% CI 1.16, 153.69, P = 0.038). In addition, a total of 733 target genes of miR-1180-3p were found from three prediction databases. The GO analyses demonstrated that the target genes were closely related to the proliferation and malignancy of tumors. The KEGG analysis showed that target genes were enriched in several key cancer-related signaling pathways, including the Pathways in cancer, the Ras signaling pathway, and the MAPK signaling pathway. In conclusion, we demonstrate that miR-1180-3p is upregulated in HCC and is associated with a poor prognosis. Thus, miR-1180-3p might be useful as a prognostic marker for HCC.
miRNAs 在人类肿瘤发生中起着不可或缺的作用。在包括肝细胞癌（HCC）在内的几种类型的癌症中，已经观察到失调的 miR-1180-3p。本研究旨在探讨 miR-1180-3p 的表达水平与 HCC 患者的临床特征和总生存率之间的相关性。使用 RT-qPCR 方法验证了从 GEO 和 TCGA 数据库中选择的 miR-1180-3p 的表达和临床意义。使用 3 个 miRNA 靶基因预测数据库获得 miR-1180-3p 的靶基因，并使用在线工具 WebGestalt 分析其功能。与非肿瘤性肝组织相比，miR-1180-3p 在 88 例 HCC 组织中表达明显上调（0.004±0.009 与 0.002±0.002，t=-2.099，P=0.038）。此外，我们发现 miR-1180-3p 的表达水平与肿瘤数量（χ²=9.157，P=0.006）和 MVI（χ²=11.354，P=0.003）显著相关。基于 Kaplan-Meier 分析，miR-1180-3p 高表达的患者总生存率短于 miR-1180-3p 低表达的患者（P=0.002）。此外，多变量 Cox 分析表明，miR-1180-3p 表达是总生存率的独立预后因素（HR=13.36，95%CI 1.16，153.69，P=0.038）。此外，从三个预测数据库中总共发现了 miR-1180-3p 的 733 个靶基因。GO 分析表明，靶基因与肿瘤的增殖和恶性密切相关。KEGG 分析表明，靶基因富集在几个关键的癌症相关信号通路中，包括癌症途径、Ras 信号通路和 MAPK 信号通路。总之，我们证明 miR-1180-3p 在 HCC 中上调，并与不良预后相关。因此，miR-1180-3p 可能可作为 HCC 的预后标志物。
