Merson A, Brochier J
Department of Haematology, Riga Medical Institute, Latvian, U.S.S.R.
Immunol Lett. 1988 Dec;19(4):269-71. doi: 10.1016/0165-2478(88)90153-8.
The peripheral blood lymphocytes from 39 patients from the Latvian S.S.R.T., U.S.S.R. with chronic lymphocytic leukaemia (CLL) have been phenotyped with various monoclonal antibodies representing the major clusters of differentiation (CD) used for phenotyping B cells. A clear delineation of two groups of patients was evidenced. The major group (33/39) possessed leukaemic cells bearing surface immunoglobulins (SIg) at a low density, Class II HLA, and CD5, CD24 and CD37 molecules but not CD21, CD22 and CD35. CD23 antigen was seen only once under microscope examination, but could be visualized by flow cytometry. CD6 antibody reacted with cells from about 1/3 of this group of patients. In the six patients of the second group the leukaemic phenotype was SIg+, Class II HLA+, CD5+, 24+, 37+, 21+, 22+, 35+, 23+ and 6-. The main finding is the concomitant expression of CD22, CD21 (CR2) and CD35 (CR1) molecules, all involved in B cell activation. It is not yet known whether these observations correlate with different clinical evolutions of the disease.
对来自苏联拉脱维亚苏维埃社会主义共和国肿瘤研究所的39例慢性淋巴细胞白血病（CLL）患者的外周血淋巴细胞，用代表用于B细胞表型分型的主要分化簇（CD）的各种单克隆抗体进行了表型分析。结果明确显示出两组患者。主要组（33/39）的白血病细胞表面免疫球蛋白（SIg）密度低，具有II类HLA以及CD5、CD24和CD37分子，但不具有CD21、CD22和CD35。在显微镜检查下仅发现1例CD23抗原阳性，但通过流式细胞术可检测到。CD6抗体与该组约1/3患者的细胞发生反应。第二组的6例患者白血病表型为SIg +、II类HLA +、CD5 +、24 +、37 +、21 +、22 +、35 +、23 +和6 -。主要发现是参与B细胞活化的CD22、CD21（CR2）和CD35（CR1）分子同时表达。这些观察结果是否与该疾病的不同临床进展相关尚不清楚。
