Laboratório de Biologia Molecular, Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil.
Laboratório de Biodefesa, Instituto de Biologia do Exército, Rio de Janeiro, RJ, Brazil.
Mem Inst Oswaldo Cruz. 2020;115:e200200. doi: 10.1590/0074-02760200200. Epub 2020 Jul 13.
Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time reverse transcriptase polymerase chain reaction (RT-PCR) (fast mode) based on Centers for Disease Control and Prevention (CDC) recommendations. First cases from Brazil, Rio de Janeiro, IBEx, and diagnosis team were reported in D36, D44, D66, and D74 respectively. Therefore, after 1300 tests, we recommend N1/N2 primer sets (CDC) for preliminary and Charité protocol confirmation in case of positive results. Moreover, every professional should be tested before starting work, in addition to weekly tests for everyone involved.
冠状病毒病（COVID-19）对全球健康构成严重威胁。本文提供了政府警报（1 月 22 日）（D）以来早期分子诊断的历史时间表。经过计算机分析，巴西陆军生物研究所（IBEx-RJ）使用基于疾病控制与预防中心（CDC）建议的实时逆转录聚合酶链反应（RT-PCR）（快速模式）对样本进行了内部测试。来自巴西、里约热内卢、IBEx 和诊断团队的首例病例分别在 D36、D44、D66 和 D74 日报告。因此，在进行了 1300 次测试后，我们建议使用 N1/N2 引物组（CDC）进行初步和 Charité 方案确认，如果结果为阳性。此外，每个专业人员在开始工作之前都应该进行测试，并且所有相关人员每周都应该进行测试。
