Buse Helen Y, Morris Brian J, Gomez-Alvarez Vicente, Szabo Jeffrey G, Hall John S
Homeland Security and Materials Management Division, Center for Environmental Solutions & Emergency Response (CESER), Office of Research and Development (ORD), US Environmental Protection Agency (USEPA), Cincinnati, OH 45268, USA.
Pegasus Technical Services, Inc c/o US EPA, Cincinnati, OH 45268, USA.
Pathogens. 2020 Jul 13;9(7):567. doi: 10.3390/pathogens9070567.
Understanding survival mechanisms within building water systems (BWSs) is challenging due to varying engineering, operational, and water quality characteristics unique to each system. This study aimed to evaluate , mycobacteria, and free-living amoebae occurrence within a BWS over 18-28 months at six locations differing in plumbing material and potable water age, quality, and usage. A total of 114 bulk water and 57 biofilm samples were analyzed. culturability fluctuated seasonally with most culture-positive samples being collected during the winter compared to the spring, summer, and fall months. Positive and negative correlations between and occurrence and other physiochemical and microbial water quality parameters varied between location and sample types. Whole genome sequencing of 19 presumptive isolates, from four locations across three time points, identified nine isolates as serogroup (sg) 1 sequence-type (ST) 1; three as sg5 ST1950 and ST2037; six as ; and one as . Results showed the presence of a diverse population with consistent and sporadic occurrence at four and two locations, respectively. Viewed collectively with similar studies, this information will enable a better understanding of the engineering, operational, and water quality parameters supporting growth within BWSs.
由于每个建筑供水系统（BWS）都有独特的工程、运行和水质特征，了解其内部的生存机制具有挑战性。本研究旨在评估在18至28个月内，六个在管道材料、饮用水使用年限、水质和用途方面存在差异的地点的BWS中分枝杆菌和自由生活阿米巴的出现情况。共分析了114份大量水样和57份生物膜样本。分枝杆菌的可培养性随季节波动，与春季、夏季和秋季相比，大多数培养阳性样本是在冬季采集的。分枝杆菌出现情况与其他理化和微生物水质参数之间的正相关和负相关在不同地点和样本类型之间有所不同。对来自三个时间点四个地点的19株推定分枝杆菌分离株进行全基因组测序，确定9株为分枝杆菌血清群（sg）1序列型（ST）1；3株为分枝杆菌sg5 ST1950和ST2037；6株为其他；1株为另一类型。结果表明，在四个地点存在多样化的分枝杆菌种群，分别在两个地点持续和零星出现。与类似研究综合来看，这些信息将有助于更好地理解支持BWS内分枝杆菌生长的工程、运行和水质参数。
