Zayed Ashraf R, Bunk Boyke, Jaber Lina, Abu-Teer Hadeel, Ali Mousa, Steinert Michael, Höfle Manfred G, Brettar Ingrid, Bitar Dina M
Eur Clin Respir J. 2023 Jan 20;10(1):2168346. doi: 10.1080/20018525.2023.2168346. eCollection 2023.
is water-based bacterium causing Legionnaires' disease (LD). We describe the first documented case of nosocomial LD caused by sequence type (ST) 461 and serogroup 6. The etiology of LD was confirmed by culturing the bronchoalveolar lavage sample retrieving strain ALAW1. A 7-days treatment of the LD patient with Azithromycin and Levofloxacin allowed complete recovery.
In details, we sequenced the whole genome of the ALAW1 using Illumina HiSeq platform. The sequence of ALAW1 was aligned with the genome sequence from the closely related reference strain Alcoy 2300/99 and a whole-genome phylogeny based on single nucleotide polymorphisms (SNPs) was created using Parsnp software. Also, the TYGS web-server was used in order to compare the genome with type strain.
An analysis of the population structure by SNP and TYGS comparison clustered ALAW1 with the reference genome Alcoy 2300/99. Blastp analysis of the type IV secretion Dot/Icm system genes showed that these genes were highly conserved with (≤25%) structural differences at the protein level.
Overall, this study provides insights into detailed genome structure and demonstrated the value of whole-genome sequencing as the ultimate typing tool for
嗜肺军团菌是一种导致军团病（LD）的水基细菌。我们描述了首例由序列类型（ST）461和血清群6引起的医院内军团病病例。通过培养支气管肺泡灌洗样本中分离出的菌株ALAW1，证实了军团病的病因。对该军团病患者进行为期7天的阿奇霉素和左氧氟沙星治疗后，患者完全康复。
具体而言，我们使用Illumina HiSeq平台对ALAW1的全基因组进行测序。将ALAW1的序列与密切相关的参考菌株Alcoy 2300/99的基因组序列进行比对，并使用Parsnp软件基于单核苷酸多态性（SNP）创建全基因组系统发育树。此外，还使用TYGS网络服务器将该基因组与模式菌株进行比较。
通过SNP分析群体结构以及与TYGS比较，将ALAW1与参考基因组Alcoy 2300/99聚类在一起。对IV型分泌Dot/Icm系统基因的Blastp分析表明，这些基因高度保守，在蛋白质水平上结构差异≤25%。
总体而言，本研究深入了解了详细的基因组结构，并证明了全基因组测序作为最终分型工具的价值。
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