聚合酶链反应和环介导等温扩增法检测伊朗阿瓦士市（伊朗西南部）公共公园土壤中的旋毛虫属。
Comparison of the Detection of Toxocara Spp. in the Soils of Public Parks of Ahvaz (Southwest of Iran) by PCR and Loop-Mediated Isothermal Amplification (LAMP).
机构信息
Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Infect Disord Drug Targets. 2021;21(3):375-383. doi: 10.2174/1871526520666200715100433.
INTRODUCTION
The infections caused by Toxocara spp. are considered as one of the most important zoonotic diseases in the world, especially in developing countries. Human toxocariasis, particularly in children, is acquired by playing in public parks. Hence, the aim of the current study was to detect Toxocara spp. in the soils of public parks of the city of Ahvaz, southwest of Iran, using the PCR and loop-mediated isothermal amplification (LAMP) methods.
METHODS
In this descriptive cross-sectional study, 260 soil samples were randomly collected from the different public parks of the city of Ahvaz. After performing zinc sulfate (ZnSO) flotation technique, the DNA samples were extracted from the isolated Toxocara spp. eggs. Lastly, the extracted DNA was used for PCR and LAMP-based molecular detection.
RESULTS
Out of 260 specimens, 57 (21.9%) samples were found positive for Toxocara spp., using the PCR method, out of that 38 (14.6%) samples were positive for T. canis and 19 (7.3%) samples were positive for T. cati. Also, out of 260 specimens, 81 (31.1%) cases were positive for Toxocara species, using the LAMP method, among them 51 (19.6%) samples were found positive for T. canis and 30 (11.5%) samples were positive for T. cati. Kappa (κ) coefficient between PCR and LAMP showed a strong agreement (0.766, P-value=0.002).
CONCLUSION
The obtained data showed a relatively high outbreak of Toxocara spp. in the public parks' soils of the city, using the PCR and LAMP methods. Since the parasite can cause human toxocariasis, particularly in children; thus, the health authorities of the city of Ahvaz and other similar cities, especially in developing countries, must pay more attention to the hygiene of the public parks' soils.
简介
旋毛虫属引起的感染被认为是世界上最重要的人畜共患病之一，特别是在发展中国家。人类旋毛虫病，特别是儿童，是通过在公园玩耍获得的。因此，本研究旨在使用 PCR 和环介导等温扩增（LAMP）方法检测伊朗西南部阿瓦兹市公园土壤中的旋毛虫属。
方法
在这项描述性的横断面研究中，随机从阿瓦兹市不同的公园采集了 260 份土壤样本。在进行硫酸锌（ZnSO）浮选技术后，从分离出的旋毛虫属卵中提取 DNA 样本。最后，从提取的 DNA 中提取 DNA 用于基于 PCR 和 LAMP 的分子检测。
结果
使用 PCR 方法，在 260 个标本中，有 57 个（21.9%）标本检测出旋毛虫属阳性，其中 38 个（14.6%）标本为犬旋毛虫阳性，19 个（7.3%）标本为猫旋毛虫阳性。此外，使用 LAMP 方法，在 260 个标本中，有 81 个（31.1%）标本检测出旋毛虫属阳性，其中 51 个（19.6%）标本为犬旋毛虫阳性，30 个（11.5%）标本为猫旋毛虫阳性。PCR 和 LAMP 之间的 Kappa（κ）系数显示出很强的一致性（0.766，P 值=0.002）。
结论
使用 PCR 和 LAMP 方法，在所研究的城市公园土壤中，旋毛虫属的爆发率相对较高。由于寄生虫会引起人类旋毛虫病，特别是在儿童中；因此，阿瓦兹市和其他类似城市的卫生当局，特别是在发展中国家，必须更加关注公园土壤的卫生。
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