Increases the Potential of Breast Cancer by Reducing the Expression of the P53 Protein.

INTRODUCTION

Breast cancer is considered the most frequent type of cancer in women with high mortality worldwide, and most importantly, it is the second most common cancer. However, some breast cancer-related risk factors remain unknown. So, the current study was designed to evaluate the effect of on the biomarkers correlated with proliferation, apoptosis, inflammation, and angiogenesis in 4T1 tumor-bearing mice infected with for the first time.

METHODS

Mice were categorized into four groups: A) control, B) treated with 4T1+ , C) treated with , and D) treated with 4T1. The expression of Ki-67 and P53 was then evaluated by using the immunohistochemical technique. In addition, the levels of transforming growth factor-β, Interferon gamma-γ, Interleukin 10, tumor necrosis factor-α and vascular endothelial growth factor as well as anti- IgG were determined using the enzyme-linked immunosorbent assay method.

RESULTS

The expression of Ki-67 was significantly increased in the 4T1+ group than control and groups ( < 0.001 and < 0.001, respectively). Moreover, a significant decrease in P53 was found in the 4T1+ group than in the control and groups ( < 0.001 and < 0.001, respectively). Also, the 4T1+ group significantly reduced the expression of P53 more than 4T1 tumor-bearing mice ( = 0.005). In addition, the 4T1+ Toxocara canis group had an increasing tumor necrosis factor-α and vascular endothelial growth factor than controls ( = 0.004 and = 0.002, respectively). Furthermore, a significant reduction in Interleukin 10 was found in the 4T1+ group than in the control group ( = 0.004).

CONCLUSION

Our findings showed that could probably increase the potential of breast cancer by reducing P53 in 4T1 tumor-bearing mice infected with more than other groups.