Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Shiqiao Road 198, Hangzhou, 310021, China.
Xiangshan Gangwan Aquatic Seeds Co., Ltd, Ningbo, 315700, China.
Sci Rep. 2020 Jul 15;10(1):11621. doi: 10.1038/s41598-020-68592-0.
Larimichthys polyactis is a commercially important marine fish species in Eastern Asia, yet very few genetic resources exist. In particular, genetic linkage maps are critical tools for genetic breeding. In this study, we generated a high resolution linkage map from a family of 110 individuals and their parents by resequencing the individuals. 3,802 effective SNPs were mapped to 24 linkage groups (LGs). The map spanned 2,567.39 cm, with an average marker interval of 0.66 cm. We used the map to conduct QTL analysis for growth traits, and found 31 markers were significantly associated with growth-related traits. Specifically, three SNPs were identified for total length, nineteen SNPs for body length, and nine SNPs for body weight. The identified SNPs could explain 15.2-22.6% of the phenotypic variation. SNPs associated with growth traits were distributed on LG6 and LG11, and candidate genes included, kif26b, bat1, gna1, gbgt1, and amfr, which may regulate growth. The linkage map and mapped QTLs would be useful for improving the quality of L. polyactis via marker-assisted selection.
黄姑鱼是东亚地区一种重要的商业性海水鱼类，但遗传资源非常有限。特别是，遗传连锁图谱是遗传育种的关键工具。在这项研究中，我们通过对 110 个个体及其父母进行重测序，从一个家系中生成了一张高分辨率的连锁图谱。3802 个有效 SNP 被映射到 24 个连锁群（LG）上。图谱全长 2567.39 厘米，平均标记间隔为 0.66 厘米。我们利用该图谱对生长性状进行了 QTL 分析，发现 31 个标记与生长相关性状显著相关。具体来说，有三个 SNP 与全长相关，19 个 SNP 与体长相关，9 个 SNP 与体重相关。鉴定出的 SNP 可以解释 15.2-22.6%的表型变异。与生长性状相关的 SNP 分布在 LG6 和 LG11 上，候选基因包括 kif26b、bat1、gna1、gbgt1 和 amfr，它们可能调节生长。该连锁图谱和定位的 QTL 将有助于通过标记辅助选择提高黄姑鱼的质量。
