Yu Xinxiu, Joshi Rajesh, Gjøen Hans Magnus, Lv Zhenming, Kent Matthew
Department of Animal and Aquacultural Sciences, Faculty of Biosciences, Norwegian University of Life Sciences, As, Norway.
National Engineering Research Centre of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, China.
Front Genet. 2022 Jan 3;12:792666. doi: 10.3389/fgene.2021.792666. eCollection 2021.
Consensus and sex-specific genetic linkage maps for large yellow croaker () were constructed using samples from an F family produced by crossing a Daiqu female and a Mindong male. A total of 20,147 single nucleotide polymorphisms (SNPs) by restriction site associated DNA sequencing were assigned to 24 linkage groups (LGs). The total length of the consensus map was 1757.4 centimorgan (cM) with an average marker interval of 0.09 cM. The total length of female and male linkage map was 1533.1 cM and 1279.2 cM, respectively. The average female-to-male map length ratio was 1.2 ± 0.23. Collapsed markers in the genetic maps were re-ordered according to their relative positions in the genome assembly to produce integrated genetic linkage maps with 9885 SNPs distributed across the 24 LGs. The recombination pattern of most LGs showed sigmoidal patterns of recombination, with higher recombination in the middle and suppressed recombination at both ends, which corresponds with the presence of sub-telocentric and acrocentric chromosomes in the species. The average recombination rate in the integrated female and male maps was respectively 3.55 cM/Mb and 3.05 cM/Mb. In most LGs, higher recombination rates were found in the integrated female map, compared to the male map, except in LG12, LG16, LG21, LG22, and LG24. Recombination rate profiles within each LG differed between the male and the female, with distinct regions indicating potential recombination hotspots. Separate quantitative trait loci (QTL) and association analyses for growth related traits in 6 months fish were performed, however, no significant QTL was detected. The study indicates that there may be genetic differences between the two strains, which may have implications for the application of DNA-information in the further breeding schemes.
利用岱衢族雌鱼与闽东族雄鱼杂交产生的F家系样本，构建了大黄鱼的一致性遗传连锁图谱和性别特异性遗传连锁图谱。通过限制性位点关联DNA测序共获得20147个单核苷酸多态性（SNP），并将其分配到24个连锁群（LG）中。一致性图谱的总长度为1757.4厘摩（cM），平均标记间隔为0.09 cM。雌性和雄性连锁图谱的总长度分别为1533.1 cM和1279.2 cM。雌性与雄性图谱长度的平均比值为1.2±0.23。根据遗传图谱中标记在基因组组装中的相对位置对折叠后的标记进行重新排序，以产生整合的遗传连锁图谱，其中9885个SNP分布在24个LG上。大多数LG的重组模式呈S形，中间重组率较高，两端重组受到抑制，这与该物种中亚端着丝粒和近端着丝粒染色体的存在相一致。整合的雌性和雄性图谱中的平均重组率分别为3.55 cM/Mb和3.05 cM/Mb。在大多数LG中，与雄性图谱相比，整合的雌性图谱中重组率更高，但LG12、LG16、LG21、LG22和LG24除外。每个LG内的重组率图谱在雄性和雌性之间存在差异，不同区域表明存在潜在的重组热点。对6月龄鱼的生长相关性状进行了单独的数量性状位点（QTL）和关联分析，然而，未检测到显著的QTL。该研究表明，这两个品系之间可能存在遗传差异，这可能对DNA信息在进一步育种方案中的应用产生影响。
