Shimuta Misa, Sugihara Izumi, Ishikawa Taro
Department of Pharmacology, The Jikei University School of Medicine, Tokyo, Japan.
Department of Systems Neurophysiology, Tokyo Medical and Dental University Graduate School of Medical and Dental Sciences, Tokyo, Japan.
Commun Biol. 2020 Jul 15;3(1):381. doi: 10.1038/s42003-020-1110-2.
The cerebellum receives signals directly from peripheral sensory systems and indirectly from the neocortex. Even a single tactile stimulus can activate both of these pathways. Here we report how these different types of signals are integrated in the cerebellar cortex. We used in vivo whole-cell recordings from granule cells and unit recordings from Purkinje cells in mice in which primary somatosensory cortex (S1) could be optogenetically inhibited. Tactile stimulation of the upper lip produced two-phase granule cell responses (with latencies of ~8 ms and 29 ms), for which only the late phase was S1 dependent. In Purkinje cells, complex spikes and the late phase of simple spikes were S1 dependent. These results indicate that individual granule cells combine convergent inputs from the periphery and neocortex and send their outputs to Purkinje cells, which then integrate those signals with climbing fiber signals from the neocortex.
小脑直接从外周感觉系统接收信号，并间接从新皮层接收信号。即使是单个触觉刺激也能激活这两条通路。在此，我们报告了这些不同类型的信号是如何在小脑皮质中整合的。我们使用了小鼠颗粒细胞的体内全细胞记录和浦肯野细胞的单位记录，其中初级体感皮层（S1）可以通过光遗传学方法被抑制。对上唇进行触觉刺激产生了两相颗粒细胞反应（潜伏期约为8毫秒和29毫秒），其中只有晚期反应依赖于S1。在浦肯野细胞中，复合峰电位和简单峰电位的晚期依赖于S1。这些结果表明，单个颗粒细胞将来自外周和新皮层的汇聚输入进行整合，并将其输出发送到浦肯野细胞，然后浦肯野细胞将这些信号与来自新皮层的攀缘纤维信号进行整合。
