• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

厚朴酚通过miR-101/Mcl-1轴抑制胰腺癌进展。

Honokiol Suppressed Pancreatic Cancer Progression via miR-101/Mcl-1 Axis.

作者信息

Wang Yishuo, Liu Zhongyong, Liu Qinrong, Han Yongguang, Zang Yuncai, Zhang Huichao, Du Xuzhao, Qin Tao, Wu Yuquan

机构信息

College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, People's Republic of China.

Henan Integrated Engineering Technology Research Center of Traditional Chinese Medicine Production, Zhengzhou, People's Republic of China.

出版信息

Cancer Manag Res. 2020 Jul 1;12:5243-5254. doi: 10.2147/CMAR.S237323. eCollection 2020.

DOI:10.2147/CMAR.S237323
PMID:32669873
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7335890/
Abstract

BACKGROUND

Pancreatic cancer is one of the most aggressive malignancies. The present study aimed to examine the anti-tumor effects of honokiol in pancreatic cancer and to explore the underlying molecular mechanisms.

MATERIALS AND METHODS

In vitro functional assays determined pancreatic cancer cell proliferation, apoptosis and invasion. Xenograft nude mice model determined the in vivo anti-cancer effects of honokiol. Luciferase reporter assay determined the interaction between miR101 and myeloid cell leukemia-1 (Mcl-1).

RESULTS

Honokiol concentration-dependently suppressed pancreatic cancer cell viability. In addition, honokiol increased the caspase-3 activity and cell apoptotic rates, induced cell cycle arrest at G0/G1 phase, and inhibited cell invasion in pancreatic cancer. Interestingly, honokiol treatment induced up-regulation of miR-101 in pancreatic cancer cells. Knockdown of miR-101 attenuated the honokiol-induced cell apoptosis and inhibition in cell invasion of pancreatic cancer cells. On the other hand, miR-101 overexpression induced cell apoptosis and inhibited cell viability and invasion in pancreatic cancer. Further mechanistic study verified that Mcl-1 was negatively regulated by miR-101, and Mcl-1 overexpression counteracted the tumor-suppressive effects of honokiol on the pancreatic cancer cells. In vivo studies showed that honokiol dose-dependently suppressed tumor growth of pancreatic cancer in the nude mice and up-regulated miR-101 expression but down-regulated Mcl-1 expression in tumor tissues.

CONCLUSION

Our data showed that honokiol suppressed pancreatic cancer progression via miR-101-Mcl-1 axis. Honokiol could be a promising candidate for cancer prevention and/or therapeutic treatment for pancreatic cancer.

摘要

背景

胰腺癌是最具侵袭性的恶性肿瘤之一。本研究旨在探讨厚朴酚对胰腺癌的抗肿瘤作用，并探索其潜在的分子机制。

材料与方法

体外功能实验测定胰腺癌细胞的增殖、凋亡和侵袭能力。异种移植裸鼠模型测定厚朴酚的体内抗癌作用。荧光素酶报告基因检测法测定miR101与髓样细胞白血病-1（Mcl-1）之间的相互作用。

结果

厚朴酚浓度依赖性地抑制胰腺癌细胞活力。此外，厚朴酚增加了caspase-3活性和细胞凋亡率，诱导细胞周期停滞于G0/G1期，并抑制胰腺癌细胞的侵袭。有趣的是，厚朴酚处理可诱导胰腺癌细胞中miR-101上调。敲低miR-101可减弱厚朴酚诱导的胰腺癌细胞凋亡及对细胞侵袭的抑制作用。另一方面，miR-101过表达可诱导胰腺癌细胞凋亡，并抑制其活力和侵袭。进一步的机制研究证实，Mcl-1受miR-101负调控，Mcl-1过表达可抵消厚朴酚对胰腺癌细胞的肿瘤抑制作用。体内研究表明，厚朴酚剂量依赖性地抑制裸鼠胰腺癌的肿瘤生长，并上调肿瘤组织中miR-101表达，但下调Mcl-1表达。

结论

我们的数据表明，厚朴酚通过miR-101-Mcl-1轴抑制胰腺癌进展。厚朴酚可能是胰腺癌预防和/或治疗的有前途的候选药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/c1104250820b/CMAR-12-5243-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/f95c8057cc22/CMAR-12-5243-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/9cb4e8b71a28/CMAR-12-5243-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/a61b73d09a86/CMAR-12-5243-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/f6f03cea9b99/CMAR-12-5243-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/095bc6968ccf/CMAR-12-5243-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/06f75a968ac1/CMAR-12-5243-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/8e1c1bb34dae/CMAR-12-5243-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/c1104250820b/CMAR-12-5243-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/f95c8057cc22/CMAR-12-5243-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/9cb4e8b71a28/CMAR-12-5243-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/a61b73d09a86/CMAR-12-5243-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/f6f03cea9b99/CMAR-12-5243-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/095bc6968ccf/CMAR-12-5243-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/06f75a968ac1/CMAR-12-5243-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/8e1c1bb34dae/CMAR-12-5243-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49a2/7335890/c1104250820b/CMAR-12-5243-g0008.jpg

相似文献

1
Honokiol Suppressed Pancreatic Cancer Progression via miR-101/Mcl-1 Axis.厚朴酚通过miR-101/Mcl-1轴抑制胰腺癌进展。
Cancer Manag Res. 2020 Jul 1;12:5243-5254. doi: 10.2147/CMAR.S237323. eCollection 2020.
2
Rosmarinic inhibits cell proliferation, invasion and migration via up-regulating miR-506 and suppressing MMP2/16 expression in pancreatic cancer.迷迭香酸通过上调 miR-506 和抑制 MMP2/16 的表达抑制胰腺癌中的细胞增殖、侵袭和迁移。
Biomed Pharmacother. 2019 Jul;115:108878. doi: 10.1016/j.biopha.2019.108878. Epub 2019 May 3.
3
LncRNA SNHG14 potentiates pancreatic cancer progression via modulation of annexin A2 expression by acting as a competing endogenous RNA for miR-613.长链非编码 RNA SNHG14 通过作为 miR-613 的竞争性内源性 RNA 调节膜联蛋白 A2 的表达促进胰腺癌进展。
J Cell Mol Med. 2019 Nov;23(11):7222-7232. doi: 10.1111/jcmm.14467. Epub 2019 Sep 12.
4
Sevoflurane Inhibited Osteosarcoma Cell Proliferation And Invasion Via Targeting miR-203/WNT2B/Wnt/β-Catenin Axis.七氟醚通过靶向miR-203/WNT2B/Wnt/β-连环蛋白轴抑制骨肉瘤细胞增殖和侵袭。
Cancer Manag Res. 2019 Nov 11;11:9505-9515. doi: 10.2147/CMAR.S225911. eCollection 2019.
5
Down-regulation of miR-320 associated with cancer progression and cell apoptosis via targeting Mcl-1 in cervical cancer.在宫颈癌中，miR-320通过靶向Mcl-1实现下调，这与癌症进展和细胞凋亡相关。
Tumour Biol. 2016 Jul;37(7):8931-40. doi: 10.1007/s13277-015-4771-6. Epub 2016 Jan 11.
6
MicroRNA-140-5p inhibits salivary adenoid cystic carcinoma progression and metastasis via targeting survivin.微小RNA-140-5p通过靶向生存素抑制涎腺腺样囊性癌的进展和转移。
Cancer Cell Int. 2019 Nov 16;19:301. doi: 10.1186/s12935-019-1018-4. eCollection 2019.
7
miR-204 mediated loss of Myeloid cell leukemia-1 results in pancreatic cancer cell death.miR-204 介导髓样细胞白血病-1 的缺失导致胰腺癌细胞死亡。
Mol Cancer. 2013 Sep 11;12(1):105. doi: 10.1186/1476-4598-12-105.
8
HOXA-AS2 Promotes Proliferation and Induces Epithelial-Mesenchymal Transition via the miR-520c-3p/GPC3 Axis in Hepatocellular Carcinoma.HOXA-AS2通过miR-520c-3p/GPC3轴促进肝癌细胞增殖并诱导上皮-间质转化
Cell Physiol Biochem. 2018;50(6):2124-2138. doi: 10.1159/000495056. Epub 2018 Nov 9.
9
MicroRNA-506 inhibits the proliferation and invasion of mantle cell lymphoma cells by targeting B7H3.MicroRNA-506 通过靶向 B7H3 抑制套细胞淋巴瘤细胞的增殖和侵袭。
Biochem Biophys Res Commun. 2019 Jan 22;508(4):1067-1073. doi: 10.1016/j.bbrc.2018.12.055. Epub 2018 Dec 12.
10
Honokiol inhibits bladder tumor growth by suppressing EZH2/miR-143 axis.厚朴酚通过抑制EZH2/miR-143轴来抑制膀胱肿瘤生长。
Oncotarget. 2015 Nov 10;6(35):37335-48. doi: 10.18632/oncotarget.6135.

引用本文的文献

1
Targeting Annexin A1 as a Druggable Player to Enhance the Anti-Tumor Role of Honokiol in Colon Cancer through Autophagic Pathway.将膜联蛋白A1作为可成药靶点，通过自噬途径增强厚朴酚在结肠癌中的抗肿瘤作用。
Pharmaceuticals (Basel). 2023 Jan 1;16(1):70. doi: 10.3390/ph16010070.
2
Apoptosis Triggering, an Important Way for Natural Products From Herbal Medicines to Treat Pancreatic Cancers.细胞凋亡触发：草药天然产物治疗胰腺癌的重要途径
Front Pharmacol. 2022 Feb 9;12:796300. doi: 10.3389/fphar.2021.796300. eCollection 2021.
3
Honokiol inhibits the growth of SKBR3 cells.

本文引用的文献

1
Anti-proliferative activity and structure-activity relationship of honokiol derivatives.霍楠醇衍生物的抗增殖活性及构效关系。
Bioorg Med Chem. 2019 Aug 15;27(16):3729-3734. doi: 10.1016/j.bmc.2019.06.042. Epub 2019 Jun 27.
2
Honokiol inhibits breast cancer cell metastasis by blocking EMT through modulation of Snail/Slug protein translation.和厚朴酚通过调节 SNAI1/SNAI2 蛋白翻译抑制 EMT 从而抑制乳腺癌细胞转移。
Acta Pharmacol Sin. 2019 Sep;40(9):1219-1227. doi: 10.1038/s41401-019-0240-x. Epub 2019 Jun 24.
3
Cytological effects of honokiol treatment and its potential mechanism of action in non-small cell lung cancer.
厚朴酚抑制SKBR3细胞的生长。
Transl Cancer Res. 2020 Dec;9(12):7596-7604. doi: 10.21037/tcr-20-3110.
4
Honokiol Suppresses Perineural Invasion of Pancreatic Cancer by Inhibiting SMAD2/3 Signaling.厚朴酚通过抑制SMAD2/3信号通路抑制胰腺癌的神经周围浸润。
Front Oncol. 2021 Oct 4;11:728583. doi: 10.3389/fonc.2021.728583. eCollection 2021.
5
Regulation of the PTEN/PI3K/AKT pathway in RCC using the active compounds of natural products .利用天然产物的活性化合物调控肾细胞癌中的 PTEN/PI3K/AKT 通路。
Mol Med Rep. 2021 Nov;24(5). doi: 10.3892/mmr.2021.12406. Epub 2021 Sep 7.
霍楠醇处理对非小细胞肺癌的细胞学作用及其潜在作用机制。
Biomed Pharmacother. 2019 Sep;117:109058. doi: 10.1016/j.biopha.2019.109058. Epub 2019 Jun 5.
4
Honokiol Induces Autophagic Apoptosis in Neuroblastoma Cells through a P53-Dependent Pathway.霍诺酚诱导神经母细胞瘤细胞通过 P53 依赖性途径发生自噬性细胞凋亡。
Am J Chin Med. 2019;47(4):895-912. doi: 10.1142/S0192415X19500472. Epub 2019 May 15.
5
MicroRNA-146b Overexpression Promotes Human Bladder Cancer Invasion via Enhancing ETS2-Mediated mmp2 mRNA Transcription.微小RNA-146b过表达通过增强ETS2介导的mmp2 mRNA转录促进人膀胱癌侵袭。
Mol Ther Nucleic Acids. 2019 Jun 7;16:531-542. doi: 10.1016/j.omtn.2019.04.007. Epub 2019 Apr 14.
6
MiR-101: a potential therapeutic target of cancers.微小RNA-101：癌症的一个潜在治疗靶点。
Am J Transl Res. 2018 Nov 15;10(11):3310-3321. eCollection 2018.
7
Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries.全球癌症统计数据 2018：GLOBOCAN 对全球 185 个国家/地区 36 种癌症的发病率和死亡率的估计。
CA Cancer J Clin. 2018 Nov;68(6):394-424. doi: 10.3322/caac.21492. Epub 2018 Sep 12.
8
MiR-101 inhibits cell proliferation and invasion of pancreatic cancer through targeting STMN1.miR-101 通过靶向 STMN1 抑制胰腺癌细胞增殖和侵袭。
Cancer Biomark. 2018;23(2):301-309. doi: 10.3233/CBM-181675.
9
MCL-1 is a prognostic indicator and drug target in breast cancer.MCL-1 是乳腺癌的预后指标和药物靶点。
Cell Death Dis. 2018 Jan 16;9(2):19. doi: 10.1038/s41419-017-0035-2.
10
Bitter melon extract inhibits breast cancer growth in preclinical model by inducing autophagic cell death.苦瓜提取物通过诱导自噬性细胞死亡在临床前模型中抑制乳腺癌生长。
Oncotarget. 2017 Aug 3;8(39):66226-66236. doi: 10.18632/oncotarget.19887. eCollection 2017 Sep 12.