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二倍体酿酒酵母经X射线照射后的分次给药及液体保存恢复。I. 对生长阶段的依赖性

Split-dose and liquid-holding recovery after X-irradiation on diploid yeast Saccharomyces cerevisiae. I. Dependence on growth-phase.

作者信息

Wienhard I, Kiefer J

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1977 May;31(5):477-84. doi: 10.1080/09553007714550571.

DOI:10.1080/09553007714550571
PMID:326710
Abstract

Liquid-holding recovery (LHR) and the sparing effect of dose fractionation were investigated with 100 kVp-X-rays in diploid yeast from exponential and stationary phase. Exponential cells showed prompt and complete split-dose recovery. The ability to undergo LHR remained constant during 2-5 hours incubation after the first dose. Stationary-phase cells did not exhibit split-dose recovery (SDR) in a simple way: there was an increase in sensitivity after 3-5 hours followed by a gradual increases with time. It is suggested that stationary cells are starved of an essential co-factor which has to be synthesized de nova.

摘要

用100 kVp的X射线对处于指数生长期和稳定期的二倍体酵母进行了液体保持恢复(LHR)和剂量分割的 sparing 效应研究。指数生长期的细胞表现出迅速且完全的分次剂量恢复。在第一次照射后2至5小时的孵育期间,进行LHR的能力保持恒定。稳定期的细胞没有以简单的方式表现出分次剂量恢复(SDR):在3至5小时后敏感性增加,随后随时间逐渐增加。有人认为,稳定期细胞缺乏一种必需的辅助因子,这种因子必须重新合成。

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