二倍体酿酒酵母经X射线照射后的分次剂量及持液恢复。II. 恢复过程对细胞蛋白质代谢的依赖性

Split-dose and liquid-holding recovery after X-irradiation in diploid yeast Saccharomyces cerevisiae. II. Dependence of the recovery process on cellular protein metabolism.

作者信息

Wienhard I, Kiefer J

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1977 Jul;32(1):33-41. doi: 10.1080/09553007714550741.

Abstract

In diploid yeast, split-dose recovery (SDR) after X-irradiation was affected, if incubation between split doses was performed in the presence of the protein-synthesis inhibitor, cycloheximide. In exponentially-growing cell-cultures, early SDR was undisturbed but complete recovery was not achieved. Concomitantly the cells show a decresing ability to perform subsequent liquid-holding recovery (LHR). In stationary-phase cell-cultures, SDR was completely suppressed. The cells show, however, recovery from potentially lethal damage in the presence of cycloheximide during incubation between the dose-fractions. The experimental results suggest that in diploid yeast SDR after X-irradiation is an enzymatic process dependent on a functioning protein metabolism.

摘要

在二倍体酵母中，如果在分次照射之间的孵育过程中存在蛋白质合成抑制剂环己酰亚胺，那么X射线照射后的分次剂量恢复（SDR）会受到影响。在指数生长的细胞培养物中，早期SDR不受干扰，但无法实现完全恢复。与此同时，细胞进行后续液体保持恢复（LHR）的能力逐渐下降。在稳定期细胞培养物中，SDR被完全抑制。然而，在剂量分割之间的孵育过程中，细胞在环己酰亚胺存在的情况下能从潜在致死损伤中恢复。实验结果表明，二倍体酵母经X射线照射后的SDR是一个依赖于正常蛋白质代谢的酶促过程。