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处于静止期和对数期的二倍体酵母培养物在受到γ射线和α射线照射后的持液恢复情况。

Liquid holding recovery in stationary and log phase cultures of diploid yeast exposed to gamma and alpha radiations.

作者信息

Reddy N M, Rao B S, Murthy M S

出版信息

Radiat Environ Biophys. 1976 Oct 7;13(3):167-75. doi: 10.1007/BF01330761.

DOI:10.1007/BF01330761
PMID:790443
Abstract

The kinetics of liquid-holding recovery (LHR) in diploid yeast after gamma and alpha irradiation is studied. In case of stationary phase culture the rate and extent of LHR is found to be greater for gamma-ray-induced damage than for alpha-ray-induced damage. At 10% survival level, the half-time for recovery is 5.2 h for gamma-ray damage and 12 h for alpha-ray damage. Further, while the recovery factor for alpha damage reaches saturation at 5% survival level, that for gamma damage continues to increase as survival level decreases. Oxygen is required for the recovery process during LH after gamma irradiation. The cells can recover to the same extent from both oxygen-dependent and oxygen-independent components of damage. Log phase cells containing a high per cent of budding cells, however, exhibit negative liquid holding effect after gamma irradiation.

摘要

研究了二倍体酵母在γ射线和α射线辐照后持液恢复(LHR)的动力学。在静止期培养的情况下,发现γ射线诱导损伤的LHR速率和程度大于α射线诱导损伤的情况。在10%存活水平时,γ射线损伤恢复的半衰期为5.2小时,α射线损伤为12小时。此外,虽然α损伤的恢复因子在5%存活水平时达到饱和,但γ损伤的恢复因子随着存活水平降低而继续增加。γ射线辐照后持液期间的恢复过程需要氧气。细胞可以从依赖氧气和不依赖氧气的损伤成分中恢复到相同程度。然而,含有高比例出芽细胞的对数期细胞在γ射线辐照后表现出负持液效应。

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本文引用的文献

1
DARK RECOVERY PHENOMENA IN YEAST. II. CONDITIONS THAT MODIFY THE RECOVERY PROCESS.酵母中的暗恢复现象。II. 影响恢复过程的条件。
Radiat Res. 1964 Dec;23:564-79.
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DARK RECOVERY PHENOMENA IN YEAST. 1. COMPARATIVE EFFECTS WITH VARIOUS INACTIVATING AGENTS.酵母中的暗恢复现象。1. 与各种灭活剂的比较效应。
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[Recovery processes in haploid yeast cells following x-irradiation].[X射线照射后单倍体酵母细胞的恢复过程]
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Differential "liquid holding recovery" for the lethal effect and cytoplasmic "petite" induction by UV light in Saccharomyces cerevisiae.酿酒酵母中紫外线对致死效应和细胞质“小菌落”诱导的差异“持液恢复”
Mol Gen Genet. 1970;109(1):69-83. doi: 10.1007/BF00334047.
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The effects of UV-light post-treatments on the survival characteristics of 21 UV-sensitive mutants of Saccharomyces cerevisiae.紫外线后处理对21株酿酒酵母紫外线敏感突变体存活特性的影响。
Mutat Res. 1969 Nov-Dec;8(3):545-57. doi: 10.1016/0027-5107(69)90072-4.
8
Similarities between a UV-sensitive mutant of yeast and bacterial mutants lacking excision-repair ability.酵母的紫外线敏感突变体与缺乏切除修复能力的细菌突变体之间的相似性。
Mol Gen Genet. 1969 Jul 3;104(3):253-7. doi: 10.1007/BF02539289.
9
Induction of heteroallelic reversions and lethality in Saccharomyces cerevisiae exposed to radiations of various LET ( 60 Co rays, heavy ions and - mesons) in air and nitrogen atmospheres.在空气和氮气环境中,将酿酒酵母暴露于不同传能线密度(60Co射线、重离子和π介子)的辐射下,诱导杂合等位基因回复突变和致死效应。
Radiat Res. 1971 Sep;47(3):635-43.
10
Dark recovery processes in Escherichia coli irradiated with ultraviolet light. I. Effect of rec mutations on liquid holding recovery.紫外线照射大肠杆菌后的暗修复过程。I. rec突变对液体保持修复的影响
J Bacteriol. 1968 Aug;96(2):365-73. doi: 10.1128/jb.96.2.365-373.1968.