Jensen L, Kuff E L, Wilson S H, Steinberg A D, Klinman D M
National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892.
J Autoimmun. 1988 Feb;1(1):73-83. doi: 10.1016/0896-8411(88)90078-9.
Sera from autoimmune patients and normal volunteers were tested for antibodies to the A1 core protein of heteronuclear ribonucleoprotein (hnRNP) particles by ELISA and Western blot assays. The A1 protein used in these studies was produced by recombinant DNA technology. Thirty-seven per cent of patients with systemic lupus erythematosus produced anti-A1 antibodies, compared to 7% of normal controls. Several strains of autoimmune mice were also analysed. They spontaneously made high-titered responses to A1. Normal strains showed very low anti-A1 response unless they were specifically immunized with the antigen. Following immunization, normal mice also made high-titered responses to the A1 protein. These studies demonstrated that the A1 protein itself is an autoantigenic component of hnRNP particles. Our studies uncovered no evidence of linkage between the production of anti-A1 and of anti-DNA antibodies.
通过酶联免疫吸附测定（ELISA）和蛋白质免疫印迹法，检测了自身免疫患者和正常志愿者血清中针对异核糖核蛋白（hnRNP）颗粒A1核心蛋白的抗体。这些研究中使用的A1蛋白是通过重组DNA技术制备的。系统性红斑狼疮患者中有37%产生了抗A1抗体，而正常对照者中这一比例为7%。还分析了几种自身免疫小鼠品系。它们自发地对A1产生高滴度反应。正常品系除非用该抗原进行特异性免疫，否则抗A1反应非常低。免疫后，正常小鼠也对A1蛋白产生高滴度反应。这些研究表明，A1蛋白本身是hnRNP颗粒的一种自身抗原成分。我们的研究未发现抗A1抗体和抗DNA抗体产生之间存在关联的证据。
