González-Villaseñor L I, Zhang P J, Chen T T, Powers D A
Department of Biology, Johns Hopkins University, Baltimore, MD 21218.
Gene. 1988 May 30;65(2):239-46. doi: 10.1016/0378-1119(88)90460-x.
A cDNA library was constructed using mRNA isolated from coho salmon pituitaries. By employing rainbow trout growth hormone cDNA as a probe, the coho salmon cDNA was isolated and the complete nucleotide (nt) sequence determined. The coding region contains 630 nt while the 5'- and 3'-untranslated regions are 64 and 489 nt in length, respectively. Comparison of the noncoding regions of coho and chum salmon cDNAs reveal identity at the 5' end but significant variation in the 3' end. Chum salmon and rainbow trout have identical amino acid (aa) sequences, but coho salmon growth hormone has a sequence that differs by 6 of the 188 predicted aa. Since salmonids are tetraploid, this difference may be the result of either divergence of the same growth hormone locus or of variation between different loci. Comparisons of the cDNA restriction maps of these three fish species suggest the former possibility.
利用从银大麻哈鱼垂体中分离出的mRNA构建了一个cDNA文库。通过使用虹鳟生长激素cDNA作为探针，分离出了银大麻哈鱼cDNA并确定了其完整的核苷酸（nt）序列。编码区包含630个nt，而5'-非翻译区和3'-非翻译区的长度分别为64和489个nt。银大麻哈鱼和马苏大麻哈鱼cDNA非编码区的比较显示，5'端具有同一性，但3'端存在显著差异。马苏大麻哈鱼和虹鳟具有相同的氨基酸（aa）序列，但银大麻哈鱼生长激素的序列在预测的188个aa中有6个不同。由于鲑科鱼类是四倍体，这种差异可能是同一生长激素基因座发生分歧或不同基因座之间存在变异的结果。这三种鱼类的cDNA限制性图谱比较表明是前一种可能性。
