黄鳍鲷生长激素cDNA的分子克隆与表达

Molecular cloning and expression of yellowfin porgy (Acanthopagrus latus houttuyn) growth hormone cDNA.

Tsai H J, Lin K L, Chen T T

Institute of Fisheries Science, National Taiwan University, Taipei, Republic of China.

Comp Biochem Physiol B. 1993 Apr;104(4):803-10. doi: 10.1016/0305-0491(93)90216-r.

Abstract

The growth hormone cDNA of yellowfin porgy (ypGH cDNA) consisted of 915 base pairs. 2. The deduced amino acid (aa) sequence showed that the pre-GH comprised 204 residues, of which the first 17 residues formed a signal peptide. 3. Comparison of aa sequence of ypGH to seabream, tuna, rainbow trout and chum salmon showed that ypGH shared 95.1, 94.1, 65.3 and 62.4% homology with these species, respectively. 4. By expressing the ypGH cDNA in E. coli, a polypeptide around 23 kilodaltons (kDa) was found which was immunoreactive to GH antibody.

摘要

黄鳍鲷生长激素cDNA（ypGH cDNA）由915个碱基对组成。2. 推导的氨基酸（aa）序列表明，前生长激素由204个残基组成，其中前17个残基形成信号肽。3. 将ypGH的氨基酸序列与鲷鱼、金枪鱼、虹鳟和马苏大麻哈鱼进行比较，结果显示ypGH与这些物种的同源性分别为95.1%、94.1%、65.3%和62.4%。4. 通过在大肠杆菌中表达ypGH cDNA，发现了一种约23千道尔顿（kDa）的多肽，它与生长激素抗体具有免疫反应性。