College of Veterinary Medicine, Chonnam National University, Gwangju, South Korea.
Andrologia. 2012 May;44 Suppl 1:796-803. doi: 10.1111/j.1439-0272.2011.01269.x. Epub 2011 Dec 26.
This study investigated the protective effects of melatonin (MLT) against doxorubicin (DXR)-induced testicular toxicity and oxidative stress in rats. DXR was given as a single intraperitoneal dose of 10 mg kg(-1) body weight to male rats at 1 h after MLT treatment on day 6 of the study. MLT at 15 mg kg(-1) body weight was administered daily by gavage for 5 days before DXR treatment followed by an additional dose for 5 days. Sperm analysis, histopathological examination and biochemical methods were used for this investigation. DXR caused a decrease in the weight of seminal vesicles, epididymal sperm count and motility and an increase in the incidence of histopathological changes of the testis. In addition, an increased malondialdehyde (MDA) concentration and decreased glutathione content, glutathione reductase (GR), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase activities were observed. On the contrary, MLT treatment significantly ameliorated DXR-induced testicular toxicity in rats. Moreover, MDA concentration and GR, GST and SOD activities were not affected when MLT was administered in conjunction with DXR. These results indicate that MLT had a protective effect against DXR-induced testicular toxicity and that the protective effects of MLT may be due to both the inhibition of lipid peroxidation and increased antioxidant activity.
本研究探讨了褪黑素（MLT）对阿霉素（DXR）诱导的大鼠睾丸毒性和氧化应激的保护作用。在研究的第 6 天，DXR 在 MLT 处理后 1 小时以 10mg/kg 体重的单次腹腔剂量给予雄性大鼠。在 DXR 治疗前 5 天每天通过灌胃给予 MLT 15mg/kg 体重，然后再给予额外的 5 天剂量。进行了精子分析、组织病理学检查和生化方法的研究。DXR 导致精囊、附睾精子计数和活力降低，睾丸组织学变化的发生率增加。此外，观察到丙二醛（MDA）浓度升高，谷胱甘肽含量、谷胱甘肽还原酶（GR）、谷胱甘肽-S-转移酶（GST）、超氧化物歧化酶（SOD）和过氧化氢酶活性降低。相反，MLT 处理显著改善了大鼠的 DXR 诱导的睾丸毒性。此外，当 MLT 与 DXR 联合给药时，MDA 浓度和 GR、GST 和 SOD 活性不受影响。这些结果表明 MLT 对 DXR 诱导的睾丸毒性具有保护作用，MLT 的保护作用可能归因于抑制脂质过氧化和增加抗氧化活性。
