Institute of Bio-Sensing Technology, University of the West of England, Frenchay Campus, Bristol BS16 1QY, United Kingdom.
Bioelectrochemistry. 2020 Dec;136:107593. doi: 10.1016/j.bioelechem.2020.107593. Epub 2020 Jul 3.
This paper reports on a comparison between nano-ZnO/CuO and nano-ZnO nitrocellulose membrane biosensors, both of which were fabricated using a simple and inexpensive sonication technique. To produce the nano-ZnO/CuO membranes, the technique involved sonication of 1% (w/v) ZnO and 1% (w/v) CuO nano-crystal colloidal suspensions, with a volume ratio of 1:2. The membranes were analysed by scanning electron microscopy and energy-dispersive spectroscopy, which showed the gradated distribution of nanoparticles in the membrane. Impedance spectroscopy demonstrated that the sonication resulted in a greater than two-fold enhancement of the output signal. Changes in impedance phase values, at a frequency of 100 Hz, were used to establish dose dependent responses for C-reactive protein (CRP). Limits of detection of 27 pg/mL for the 1% (w/v) nano-ZnO and 16 pg/mL for the 1% (w/v) nano-ZnO/CuO nitrocellulose membrane biosensors were demonstrated.
本文报告了纳米 ZnO/CuO 和纳米 ZnO 硝酸纤维素膜生物传感器的比较，这两种传感器都是使用简单且廉价的超声技术制备的。为了制备纳米 ZnO/CuO 膜，该技术涉及超声处理 1%（w/v）的 ZnO 和 1%（w/v）CuO 纳米晶胶体悬浮液，体积比为 1:2。通过扫描电子显微镜和能量色散光谱分析，显示了纳米粒子在膜中的分级分布。阻抗谱表明，超声处理使输出信号增强了两倍以上。在 100 Hz 的频率下，阻抗相位值的变化用于建立 C 反应蛋白（CRP）的剂量依赖性响应。对于 1%（w/v）纳米 ZnO 和 1%（w/v）纳米 ZnO/CuO 硝酸纤维素膜生物传感器，检测限分别为 27 pg/mL 和 16 pg/mL。
