Institute of Preventative Medicine and Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, School of Medicine, Ningbo University, Ningbo, China.
Center of Safety and Evaluation of Drugs, Heilongjiang University of Chinese Medicine, Harbin, China.
Asia Pac J Clin Nutr. 2020;29(2):395-403. doi: 10.6133/apjcn.202007_29(2).0022.
This study aimed to explore the changes of gut bacteria in bladder cancer patients.
Newly diagnosed bladder cancer patients were recruited. All participants completed a questionnaire about personal behavior and diet. Pyrosequencing of the total genomic DNA extracted from human feces was carried out by Illumina HiSeq 2000. The copy number of target DNA for bacteria was determined by real-time quantitative PCR assay. Fecal short chain fatty acids contents were measured by gas chromatography (GC) analysis. The concentrations of lipopolysaccharide and D-lactic acid in serum were determined by enzyme-linked immunosorbent assay kits.
Fruit intake was significantly lower than in healthy controls. The numbers of Clostridium cluster XI and Prevotella in bladder cancer patients decreased. The numbers of domain bacteria and Prevotella were significantly and positively associated with fruit intake (r=0.002, p<0.05 for domain bacteria; r=0.004, p<0.05 for Prevotella). The concentration of butyric acid decreased significantly in bladder cancer patients, and the quantities of fecal butyric acid were significantly and positively associated with fruit intake (r=0.610, p<0.01). The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
本研究旨在探讨膀胱癌患者肠道细菌的变化。
招募新诊断的膀胱癌患者。所有参与者完成了一份关于个人行为和饮食的问卷。通过 Illumina HiSeq 2000 对从人粪便中提取的总基因组 DNA 进行焦磷酸测序。通过实时定量 PCR 测定细菌目标 DNA 的拷贝数。通过气相色谱（GC）分析测定粪便短链脂肪酸含量。通过酶联免疫吸附试剂盒测定血清中脂多糖和 D-乳酸的浓度。
水果摄入量明显低于健康对照组。膀胱癌患者中 Clostridium cluster XI 和 Prevotella 的数量减少。域细菌和 Prevotella 的数量与水果摄入量呈显著正相关（域细菌 r=0.002，p<0.05；Prevotella r=0.004，p<0.05）。膀胱癌患者中丁酸浓度明显降低，粪便丁酸含量与水果摄入量呈显著正相关（r=0.610，p<0.01）。两种肠道通透性敏感标志物——脂多糖和 D-乳酸的浓度在膀胱癌患者中更高。
膀胱癌患者存在肠道微生物群失调、丁酸浓度降低和肠道结构完整性受损，这可能与水果摄入不足有关。
