Xu Yuping, Wang Yongchun, Zhao Huizhang, Wu Mingde, Zhang Jing, Chen Weidong, Li Guoqing, Yang Long
State Key Laboratory of Agricultural Microbiology and Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan 430070, China.
U.S. Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA 99164, USA.
Microorganisms. 2020 Jul 14;8(7):1045. doi: 10.3390/microorganisms8071045.
The basic leucine zipper (bZIP) proteins family is one of the largest and most diverse transcription factors, widely distributed in eukaryotes. However, no information is available regarding the gene family in , an important biocontrol agent of the plant pathogen . In this study, we identified 34 genes from the genome, which were classified into 8 groups based on their phylogenetic relationships. Intron analysis showed that 28 genes harbored a variable number of introns, and 15 of them shared a feature that intron inserted into the bZIP domain. The intron position in bZIP domain was highly conserved, which was related to recognize the arginine (R) and could be treated as a genomic imprinting. Expression analysis of the genes in response to abiotic stresses indicated that they might play distinct roles in abiotic stress responses. Results showed that 22 genes were upregulated during the later stage of conidial development. Furthermore, transcriptome analysis indicated that genes are involved in different stages of mycoparasitism. Among deletion mutants of four s (07, -09, -13, and -16), only Δ mutants significantly reduced its tolerance to the oxidative stress. The other mutants exhibited no significant effects on colony morphology, mycelial growth, conidiation, and mycoparasitism. Taken together, our results suggested that genes play important roles in the abiotic stress responses, conidial development, and mycoparasitism. These results provide comprehensive information of the gene family and lay the foundation for further research on the gene family regarding their biological functions and evolutionary history.
碱性亮氨酸拉链（bZIP）蛋白家族是最大且最多样化的转录因子家族之一，广泛分布于真核生物中。然而，关于植物病原菌的重要生防菌中该基因家族的信息尚无报道。在本研究中，我们从该基因组中鉴定出34个基因，根据它们的系统发育关系将其分为8组。内含子分析表明，28个基因含有数量可变的内含子，其中15个具有内含子插入bZIP结构域的特征。bZIP结构域中的内含子位置高度保守，这与识别精氨酸（R）有关，可被视为一种基因组印记。对这些基因响应非生物胁迫的表达分析表明，它们可能在非生物胁迫响应中发挥不同作用。结果显示，22个基因在分生孢子发育后期上调。此外，转录组分析表明这些基因参与了菌寄生的不同阶段。在四个基因（07、-09、-13和-16）的缺失突变体中，只有Δ突变体显著降低了其对氧化胁迫的耐受性。其他突变体对菌落形态、菌丝生长、产孢和菌寄生均无显著影响。综上所述，我们的结果表明这些基因在非生物胁迫响应、分生孢子发育和菌寄生中发挥重要作用。这些结果提供了该基因家族的全面信息，为进一步研究该基因家族的生物学功能和进化历史奠定了基础。
