Al Shaqsi Noura Hamed Khalifa, Al Hoqani Horiya Ali Said, Hossain Mohammad Amzad, Al Sibani Mohammed Abdullah
Department of Biological Sciences and Chemistry, College of Arts and Science, University of Nizwa, P.O. Box 33, Postal Code 616, Nizwa, Oman.
School of Pharmacy, College of Pharmacy and Nursing, University of Nizwa, P.O. Box 33, Postal Code 616, Nizwa, Oman.
Biochem Biophys Rep. 2020 Jul 9;23:100779. doi: 10.1016/j.bbrep.2020.100779. eCollection 2020 Sep.
Chitin is an organic polymer and it is the most frequent marine natural polysaccharide after cellulose. The main natural sources of chitin are exoskeletons of insects, mollusks, the cell walls of certain fungi and crustaceans such as crabs, shrimps and lobsters. The waste of these marine exoskeletons are pollutant for the environment, but these waste raw materials could be useful for production of commercial products like chitin. Chitin is an important raw material used for water treatment, agricultural, biomedical, biotechnological purposes, food and paper industry and cosmetics. Based on the variety of importance, the present targets of this study are to optimize the demineralization process for the removal of calcium and phosphate contents from the waste of () by using acid at ambient temperature and to characterize the isolated demineralized sample as well as the percentage of remaining calcium and phosphorus contents by using Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES). The prepared waste carbs coarse powder samples of were demineralized with seven different concentrations of hydrochloric acid at ambient temperature for 1 h. All the demineralization samples by the different concentrations were analyzed by using sensitive ICP-OES. The results based on ICP-OES showed that among the seven different concentrations used in the demineralization process for the isolation of chitin, the best was 2 M of HCl concentration for the production of chitin. The results also showed that the optimized concentration 2 M HCl gave the minimum concentration of calcium and phosphorus compared to other concentrations applied in this experiment. In conclusion, the optimized concentration for demineralization process could be used commercially for the isolation or commercial production of chitin for agricultural, biomedical and biotechnological purposes.
几丁质是一种有机聚合物，是仅次于纤维素的最常见的海洋天然多糖。几丁质的主要天然来源是昆虫、软体动物的外骨骼，某些真菌的细胞壁以及螃蟹、虾和龙虾等甲壳类动物。这些海洋外骨骼的废弃物对环境是污染物，但这些废弃原材料可用于生产几丁质等商业产品。几丁质是用于水处理、农业、生物医学、生物技术、食品和造纸工业以及化妆品的重要原材料。基于其多种重要性，本研究目前的目标是在常温下使用酸优化脱矿质过程，以去除（）废弃物中的钙和磷含量，并使用电感耦合等离子体发射光谱法（ICP-OES）对分离出的脱矿质样品以及剩余钙和磷含量的百分比进行表征。将制备好的（）废弃碳水化合物粗粉样品在常温下用七种不同浓度的盐酸脱矿质1小时。通过使用灵敏的ICP-OES对不同浓度的所有脱矿质样品进行分析。基于ICP-OES的结果表明，在用于分离几丁质的脱矿质过程中使用的七种不同浓度中，用于生产几丁质的最佳盐酸浓度是2M。结果还表明，与本实验中使用的其他浓度相比，优化浓度2M盐酸的钙和磷浓度最低。总之，脱矿质过程的优化浓度可用于商业上分离或商业生产用于农业、生物医学和生物技术目的的几丁质。
