Applied Analytical Chemistry & Teaching and Research Center for Separation, University of Duisburg-Essen, Universitaetsstr. 5, 45141, Essen, Germany.
Analytical Chemistry - Biointerfaces, Ruhr University Bochum, Universitaetsstr. 150, 44780, Bochum, Germany.
Anal Bioanal Chem. 2020 Sep;412(23):5853-5861. doi: 10.1007/s00216-020-02810-6. Epub 2020 Jul 16.
A GCxGC-MS system was employed with a non-polar × mid-polar column set for the metabolic non-target analysis of Cobetia marina, the model bacteria for marine biofouling. C. marina was treated with ozone to investigate the intracellular metabolic state change under oxidative stress. A minimal inhibitory concentration test was involved to guarantee that the applied ozone dosages were not lethal for the cells. In this study, non-target analyses were performed to identify the metabolites according to the NIST database. As a result, over 170 signals were detected under normal living conditions including 35 potential metabolites. By the comparison of ozone-treated and non-treated samples, five compounds were selected to describe observed trends of signals in the contour plots. Oleic acid exhibited a slight growth by increasing ozone dosage. In contrast, other metabolites such as the amino acid L-proline showed less abundance after ozone treatment, which was more evident once ozone dosage was raised. Thus, this work could provide a hint for searching for up/downregulating factors in such environmental stress conditions for C. marina. Graphical abstract.
采用气相色谱-气相色谱-质谱联用系统，使用非极性×中极性柱组对海洋生物污垢模型细菌 Cobetia marina 进行代谢物非靶向分析。用臭氧处理 C. marina ，以研究氧化应激下细胞内代谢状态的变化。最小抑菌浓度试验用于保证所施加的臭氧剂量对细胞没有致死作用。在这项研究中，根据 NIST 数据库进行非靶向分析以鉴定代谢物。结果，在正常生活条件下检测到超过 170 个信号，包括 35 个潜在代谢物。通过比较臭氧处理和未处理的样品，选择了五个化合物来描述轮廓图中信号的观察趋势。油酸随着臭氧剂量的增加而略有增加。相比之下，其他代谢物，如氨基酸 L-脯氨酸，在臭氧处理后丰度降低，一旦臭氧剂量增加，这种情况就更加明显。因此，这项工作可以为寻找 Cobetia marina 在这种环境应激条件下的上调/下调因素提供线索。
