Interfibio Research Group, Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Paı̈sos Catalans 26, 43007, Tarragona, Spain.
Department of Chemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah, 21589, Saudi Arabia.
Mikrochim Acta. 2020 Jul 16;187(8):452. doi: 10.1007/s00604-020-04414-4.
The development of a gold nanoparticle aptamer assay is persued for rapid and sensitive determination of histamine in foodstuffs, which could be deployed for on-site use. The assay is based on a histamine-specific aptamer and gold nanoparticles and the salt-induced aggregation of the particles in the presence of histamine indicated by the color change from red to blue. Gold nanoparticle size, salt type, and concentration as well as aptamer concentration were optimized, and using optimum conditions, a limit of detection of 8 nM (~ 0.05 mg/kg) was obtained. Finally, the aptamer AuNP assay was applied to the determination of histamine in quality control fish samples. The histamine levels of these samples had previously been determined using HPLC and commercial ELISA kits by numerous independent laboratories and a good correlation was obtained. The developed AuNP assay is rapid, sensitive, and reproducible. Graphical abstract.
正在开发一种基于金纳米颗粒适体的测定方法，用于快速灵敏地检测食品中的组氨酸，该方法可用于现场使用。该测定方法基于组氨酸特异性适体和金纳米颗粒，并且在存在组氨酸的情况下，由于盐诱导的颗粒聚集，通过颜色从红色变为蓝色来指示。优化了金纳米颗粒的大小、盐的类型和浓度以及适体的浓度，并且在最佳条件下，检测限为 8 nM（~0.05 mg/kg）。最后，将适体 AuNP 测定法应用于质控鱼样品中组氨酸的测定。这些样品的组氨酸水平先前已通过 HPLC 和商业 ELISA 试剂盒由多个独立实验室进行了测定，并获得了良好的相关性。开发的 AuNP 测定法快速、灵敏且重现性好。
