Division of Nutritional Science, Cornell University, Ithaca, NY, USA.
J Nutr. 2020 Sep 1;150(9):2412-2418. doi: 10.1093/jn/nxaa190.
Human muscle progenitor cell (hMPC) function facilitates skeletal muscle regeneration and is influenced by circulating factors. Yet it is unknown whether dietary interventions impact hMPC function. Blueberry consumption was examined due to the pro-proliferative and antioxidant effects of blueberries and blueberry-derived compounds.
This study measured indicators of hMPC function in young and old cultures treated with serum collected from a blueberry-enriched diet (BED) intervention.
Younger (21-40 y, n = 12) and older (60-79 y, n = 10) women consumed a 6-wk BED (38 g of freeze-dried blueberries daily). Fasting serum was collected at 0, 4, and 6 wk, and a fed serum sample at 1.5 h (acute) after starting the BED intervention. Young and old hMPCs, derived from 3-5 distinct donors (biological replicates), were individually cultured in media containing pooled, age-group-matched serum from each time point. Determinants of hMPC function (e.g., hMPC number, oxidative stress resistance, and upregulation of metabolic pathways) were measured and compared within age groups.
Culturing young hMPCs in acute (compared with 0 wk) BED serum did not alter hMPC number or oxidative stress-induced cell death, but increased cellular oxygen consumption (29%, P = 0.026). Culturing young hMPCs in 6-wk (compared with 0-wk) BED serum increased hMPC number (40%, P = 0.0024), conferred minor resistance to oxidative stress-induced cell death (12.6 percentage point decrease, P = 0.10), and modestly increased oxygen consumption (36%, P = 0.09). No beneficial effect of the acute or long-term BED serum was observed in old hMPCs.
In younger women, dietary interventions could be a feasible strategy to improve hMPC function and thus muscle regeneration, through altering the serum environment.This study was registered at clinicaltrials.gov (NCT04262258).
人类肌肉祖细胞（hMPC）的功能促进了骨骼肌的再生，并且受到循环因子的影响。然而，目前尚不清楚饮食干预是否会影响 hMPC 的功能。由于蓝莓及其衍生化合物具有促增殖和抗氧化作用，因此研究了蓝莓的摄入情况。
本研究测量了在富含蓝莓饮食（BED）干预下接受血清处理的年轻和年老培养物中 hMPC 功能的各项指标。
较年轻（21-40 岁，n=12）和较年长（60-79 岁，n=10）的女性连续 6 周每天食用 38 克冻干蓝莓。在 0、4 和 6 周时采集禁食血清，并在开始 BED 干预后 1.5 小时（急性）采集进食血清样本。将源自 3-5 个不同供体（生物学重复）的年轻和年老的 hMPC 分别在含有来自每个时间点的年龄匹配的混合血清的培养基中进行培养。在年龄组内比较了 hMPC 功能（例如 hMPC 数量、氧化应激抗性和代谢途径的上调）的决定因素。
在急性（与 0 周相比）BED 血清中培养年轻的 hMPC 并未改变 hMPC 数量或氧化应激诱导的细胞死亡，但增加了细胞耗氧量（29%，P=0.026）。在 6 周（与 0 周相比）BED 血清中培养年轻的 hMPC 增加了 hMPC 数量（40%，P=0.0024），对氧化应激诱导的细胞死亡有较小的抗性（减少 12.6 个百分点，P=0.10），并适度增加了耗氧量（增加 36%，P=0.09）。在年老的 hMPC 中，急性或长期 BED 血清均未表现出有益作用。
在年轻女性中，通过改变血清环境，饮食干预可能是一种可行的策略，可以改善 hMPC 的功能，从而促进肌肉再生。本研究在 clinicaltrials.gov 注册（NCT04262258）。
