Division of Perioperative Medicine and Intensive Care, Section of Cardiothoracic Surgery and Anesthesiology, Karolinska University Hospital, Stockholm, Sweden; Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.
Department of Microbiology, Tumor and Cell Biology, Division of Clinical Microbiology, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden.
J Hosp Infect. 2020 Sep;106(1):155-162. doi: 10.1016/j.jhin.2020.07.011. Epub 2020 Jul 15.
Medical devices face the challenge of microbial biofilm attached to the surface. Ultimately, this may jeopardize the function of the device and increase the patient's risk of infection. However, reliable methods to prevent biofilm are lacking.
To investigate the effect of silicone oil-coated polypropylene plastic, used in a new automatic urinometer, on biofilm formation; furthermore, to explore the impact of silicone oil viscosity and compare polypropylene with polystyrene, another common medical plastic.
Common pathogens, including extended-spectrum beta lactamase (ESBL) -producing and multi-drug-resistant bacteria, as well as Candida albicans, were investigated. Isogenic Escherichia coli strains deficient in the important biofilm forming factors curli, cellulose and type 1 fimbriae (fim D) were used to determine the possible mode of action by silicone oil. Clear flat-bottomed polypropylene or polystyrene wells were pretreated with either low- or medium-viscosity silicone oil and microbes were added. After 72 h, biofilm formation was quantified using crystal violet assay.
Silicone oil-coated polypropylene plastic surfaces, regardless of the oil viscosity, significantly inhibited biofilm formation of all tested Gram-negative and Gram-positive bacteria, including ESBL-producing and multi-drug resistant strains, as well as C. albicans. Silicone oil did not affect bacterial or candida growth and curli fimbriae were found to be the main target of silicone oil. Polypropylene plastic itself without oil had a better effect in preventing biofilm formation than polystyrene.
These findings suggest a new strategy to decrease microbial biofilm formation, which may reduce hospital-acquired infections and prevent dysfunction of medical devices.
医疗器械面临着表面附着微生物生物膜的挑战。最终，这可能会危及设备的功能，并增加患者感染的风险。然而，目前缺乏可靠的预防生物膜的方法。
研究新型自动尿液比浊计中使用的涂有硅油的聚丙烯塑料对生物膜形成的影响；此外，还探索了硅油粘度的影响，并比较了聚丙烯与另一种常见医用塑料聚苯乙烯。
研究了包括产超广谱β-内酰胺酶（ESBL）和多药耐药菌在内的常见病原体，以及白色念珠菌。使用缺乏重要生物膜形成因子卷曲、纤维素和 1 型菌毛（fimD）的同源大肠杆菌菌株，以确定硅油的可能作用模式。用低粘度或中粘度硅油预处理透明平底聚丙烯或聚苯乙烯孔，并加入微生物。72 小时后，用结晶紫法定量测定生物膜形成。
无论硅油粘度如何，涂有硅油的聚丙烯塑料表面都能显著抑制所有测试的革兰氏阴性和革兰氏阳性细菌，包括产 ESBL 和多药耐药菌以及白色念珠菌的生物膜形成。硅油不会影响细菌或念珠菌的生长，并且发现卷曲菌毛是硅油的主要靶标。没有涂油的聚丙烯塑料本身在防止生物膜形成方面比聚苯乙烯效果更好。
这些发现为减少微生物生物膜形成提供了一种新策略，可能会降低医院获得性感染并防止医疗器械功能障碍。
