Graduate School of Environmental Science, Hokkaido University, Sapporo, 060-0810, Japan; Department of Environmental Science and Disaster Management, Noakhali Science and Technology University, Bangladesh.
Graduate School of Health Sciences, Hokkaido University, Sapporo, 060-0812, Japan.
Food Chem Toxicol. 2020 Oct;144:111577. doi: 10.1016/j.fct.2020.111577. Epub 2020 Jul 15.
Arsenic is a well-known potent toxicant affecting people by causing various human diseases. Long-term exposure to arsenic has strong adverse health effects on liver and kidney disorders, and various forms of cancer. Contrarily, curcumin and D-pinitol are bioactive dietary compounds that have antioxidant properties. Both are used to treat a broad variety of human diseases. Thus, we hypothesized that both may have synergistic effects against arsenic-induced toxicity in PC12 cells. Cells were pretreated with curcumin (1, 2.5, 5 and 10 μM), D-pinitol (1, 2.5, 5 and 10 μM) alone or in combination, then exposed to sodium arsenite (10 μM). The final concentration of curcumin 2.5 μM and D-pinitol 5 μM was selected for combination treatment based on their highest protection at lowest concentration against arsenic toxicity. Results demonstrated that pretreatment of curcumin and D-pinitol and their combined treatment with arsenic rescued PC12 cells. Western blot analysis results showed that pretreatment of curcumin and D-pinitol and their combined treatment with arsenic significantly inhibited arsenic-induced cell death through up-regulation of pro-survival proteins and down-regulation of cell death-related proteins, although these protein expressions were negatively regulated by arsenic. Furthermore, the effect of combined treatment with curcumin and D-pinitol was stronger than individual treatment.
砷是一种众所周知的有毒物质，会通过引起各种人类疾病对人体造成各种伤害。长期接触砷会对肝、肾功能紊乱和各种形式的癌症产生强烈的不良健康影响。相反，姜黄素和 D-松醇是具有抗氧化特性的生物活性膳食化合物。两者都被用于治疗多种人类疾病。因此，我们假设两者可能对 PC12 细胞中的砷诱导毒性具有协同作用。细胞先用姜黄素（1、2.5、5 和 10μM）、D-松醇（1、2.5、5 和 10μM）单独或联合预处理，然后暴露于亚砷酸钠（10μM）中。基于对砷毒性的最低浓度下的最高保护作用，选择姜黄素 2.5μM 和 D-松醇 5μM 的最终浓度进行联合处理。结果表明，姜黄素和 D-松醇的预处理以及它们与砷的联合处理可挽救 PC12 细胞。Western blot 分析结果表明，姜黄素和 D-松醇的预处理以及它们与砷的联合处理通过上调生存蛋白和下调细胞死亡相关蛋白显著抑制了砷诱导的细胞死亡，尽管这些蛋白表达受到砷的负调控。此外，姜黄素和 D-松醇联合处理的效果强于单独处理。
