定量分析滤泡上皮细胞中 EGFR 的定位和运输动态。
Quantitative analyses of EGFR localization and trafficking dynamics in the follicular epithelium.
机构信息
Center for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08103, USA.
New Jersey Center for Science, Technology & Mathematics, Kean University, Union, NJ 07083, USA.
出版信息
Development. 2020 Aug 14;147(15):dev183210. doi: 10.1242/dev.183210.
To bridge the gap between qualitative and quantitative analyses of the epidermal growth factor receptor (EGFR) in tissues, we generated an sfGFP-tagged EGF receptor (EGFR-sfGFP) in The homozygous fly appears similar to wild type with EGFR expression and activation patterns that are consistent with previous reports in the ovary, early embryo, and imaginal discs. Using ELISA, we quantified an average of 1100, 6200 and 2500 receptors per follicle cell (FC) at stages 8/9, 10 and ≥11 of oogenesis, respectively. Interestingly, the spatial localization of the EGFR to the apical side of the FCs at early stages depended on the TGFα-like ligand Gurken. At later stages, EGFR localized to basolateral positions of the FCs. Finally, we followed the endosomal localization of EGFR in the FCs. The EGFR colocalized with the late endosome, but no significant colocalization of the receptor was found with the early endosome. The EGFR-sfGFP fly is an exciting new resource for studying cellular localization and regulation of EGFR in tissues.
为了弥合组织中表皮生长因子受体（EGFR）的定性和定量分析之间的差距，我们在果蝇中生成了一个 sfGFP 标记的 EGFR（EGFR-sfGFP）。纯合子果蝇的外观类似于野生型，其 EGFR 的表达和激活模式与先前在卵巢、早期胚胎和体节盘的报道一致。使用 ELISA，我们分别在卵发生的 8/9 期、10 期和≥11 期，量化了每个滤泡细胞（FC）平均有 1100、6200 和 2500 个受体。有趣的是，EGFR 在早期阶段定位于 FC 的顶侧的空间定位取决于 TGFα 样配体 Gurken。在后期阶段，EGFR 定位于 FC 的基底外侧位置。最后，我们观察了 FC 中 EGFR 的内体定位。EGFR 与晚期内体共定位，但未发现受体与早期内体有明显的共定位。EGFR-sfGFP 果蝇是研究组织中 EGFR 的细胞定位和调节的一个令人兴奋的新资源。
Zotero 插件