Genome Research Center for Immune Disorders, School of Medicine, Wonkwang University, Iksan, Korea.
Int Arch Allergy Immunol. 2011;155(2):106-10. doi: 10.1159/000319804. Epub 2010 Dec 22.
Individual differences in susceptibility to asthma would be expected because of common DNA variants of single nucleotide polymorphisms (SNPs) across populations. The pro-inflammatory cytokine IL-17F has homology with the IL-17 motif and induces the expression of other inflammatory cytokines in airway epithelial cells. This study aimed to identify IL-17F gene polymorphisms and to determine a possible association between these polymorphisms and susceptibility to asthma through a case-control study in a Korean population.
We identified SNPs in the IL-17F gene by sequencing. Genotyping was conducted using the high-resolution melting (HRM) method on 424 asthma patients and 548 healthy controls.
The genotype and allele frequencies of rs1889570 SNP were significantly different between asthma patients and healthy controls (p = 0.001 and 0.002, respectively). The rs1889570 SNP genotype was also positively associated with the number of peripheral blood eosinophils in asthma patients (p = 0.03). The frequencies of haplotypes AA (p = 0.01), GG (p = 0.01) and AG (p = 0.006) were significantly different between asthma patients and healthy controls.
In this study, we confirmed that the rs1889570 polymorphism of the IL-17F gene is associated with susceptibility to asthma in a Korean population.
由于人群中存在单核苷酸多态性（SNP）的常见 DNA 变体，因此预计哮喘易感性存在个体差异。促炎细胞因子 IL-17F 与 IL-17 基序具有同源性，并在气道上皮细胞中诱导其他炎症细胞因子的表达。本研究旨在通过在韩国人群中进行病例对照研究，鉴定 IL-17F 基因多态性，并确定这些多态性与哮喘易感性之间的可能关联。
我们通过测序鉴定了 IL-17F 基因中的 SNP。使用高分辨率熔解（HRM）方法对 424 名哮喘患者和 548 名健康对照者进行基因分型。
rs1889570 SNP 的基因型和等位基因频率在哮喘患者和健康对照组之间存在显著差异（p = 0.001 和 0.002）。rs1889570 SNP 基因型也与哮喘患者外周血嗜酸性粒细胞计数呈正相关（p = 0.03）。哮喘患者和健康对照组之间 AA（p = 0.01）、GG（p = 0.01）和 AG（p = 0.006）的单倍型频率存在显著差异。
在这项研究中，我们证实了 IL-17F 基因的 rs1889570 多态性与韩国人群中哮喘的易感性有关。
