Department of Chemistry, National Central University, Chung-Li 320, Taiwan.
Department of Chemistry, National Central University, Chung-Li 320, Taiwan; Department of Chemical Engineering, Army Academy ROC, Chung-Li 320, Taiwan.
Food Chem. 2020 Dec 15;333:127516. doi: 10.1016/j.foodchem.2020.127516. Epub 2020 Jul 10.
Benzotriazoles (BTRs) and benzothiazoles (BTs) are two groups of emerging concern and high production volume contaminants. Via the biomagnification of the food web, they could jeopardize human health. In this work, rapid determining the presence of five BTRs and two BTs in marketed fish was performed by a novel double-vortex-ultrasonic assisted matrix solid-phase dispersion (DVUA-MSPD) and UHPLC-electrospray ionization (+)-quadrupole time-of-flight mass spectrometry detection. Unlike traditional MSPD, we simplified the method without the use of mortar/pestle and SPE-column procedures. The DVUA-MSPD factors were screened by a multilevel categorical design, and then optimized by Box-Behnken Design plus with response surface methodology. The limits of quantification were 0.15-2 ng g (dry weight). The satisfactory average recovery ranged from 70% to 93% with RSDs less than 9%. The developed method was successfully applied for the rapid determination of selected BTRs and BTs in fish samples at trace-level.
苯并三唑（BTRs）和苯并噻唑（BTs）是两组新兴关注和高产量的污染物。通过食物链的生物放大作用，它们可能会危害人类健康。在这项工作中，通过一种新颖的双涡旋超声辅助基质固相分散（DVUA-MSPD）和超高效液相色谱-电喷雾电离（+）-四极杆飞行时间质谱检测，快速检测了市售鱼类中五种 BTRs 和两种 BTs 的存在。与传统的 MSPD 不同，我们简化了方法，无需使用研钵/研杵和 SPE 柱程序。通过多级分类设计筛选 DVUA-MSPD 因素，然后使用 Box-Behnken 设计加响应面法进行优化。定量限为 0.15-2ng g（干重）。令人满意的平均回收率在 70%至 93%之间，RSD 小于 9%。该方法成功应用于痕量水平下鱼样中选定 BTRs 和 BTs 的快速测定。
