Yang Chun-Ju, Chung Wu-Hsun, Ding Wang-Hsien
Department of Chemistry, National Central University Chung-Li 320 Taiwan
Department of Chemical Engineering, Army Academy ROC Chung-Li 320 Taiwan.
RSC Adv. 2020 Sep 25;10(58):35557-35564. doi: 10.1039/d0ra05658h. eCollection 2020 Sep 21.
The extensive use of preservatives during the growth, transport and storage of vegetables has been a concern because of their known or suspected toxicity that jeopardizes human health. This paper reports the development of a technique that rapidly determines the presence of five paraben preservative residues in leafy vegetables using double-vortex-assisted matrix solid-phase dispersion (DVA-MSPD) and UHPLC-electrospray ionization(-)-quadrupole time-of-flight mass spectrometry detection. We simplified the original MSPD technique by eliminating the use of mortar/pestle and SPE-column procedures. The DVA-MSPD factors were screened by a multilevel categorical design, and then optimized by Box-Behnken Design plus response surface methodology. The limits of quantification were 1.2-1.8 ng g (dry weight). The satisfactory average recoveries were 85-104% with RSDs less than 10%. The developed method was successfully employed for the rapid determination of selected paraben residues at trace-level in leafy vegetable samples.
由于已知或怀疑防腐剂具有危害人类健康的毒性，蔬菜在生长、运输和储存过程中大量使用防腐剂一直备受关注。本文报道了一种利用双涡旋辅助基质固相分散（DVA-MSPD）和超高效液相色谱-电喷雾电离（-）-四极杆飞行时间质谱检测技术快速测定叶菜类蔬菜中五种对羟基苯甲酸酯类防腐剂残留的方法。我们通过省去研钵/研杵和固相萃取柱程序简化了原始的MSPD技术。采用多级分类设计筛选DVA-MSPD因素，然后通过Box-Behnken设计加响应面法进行优化。定量限为1.2-1.8 ng g（干重）。平均回收率令人满意，为85-104%，相对标准偏差小于10%。所建立的方法成功用于快速测定叶菜类蔬菜样品中痕量水平的选定对羟基苯甲酸酯类残留。
