Suppr超能文献

野生型大肠杆菌生产乙二醇和甘醇酸。

Ethylene glycol and glycolic acid production by wild-type Escherichia coli.

机构信息

Lab of Biorefinery, Chinese Academy of Sciences, Shanghai Advanced Research Institute, Pudong, Shanghai, People's Republic of China.

School of Life Science and Technology, ShanghaiTech University, Shanghai, People's Republic of China.

出版信息

Biotechnol Appl Biochem. 2021 Aug;68(4):744-755. doi: 10.1002/bab.1987. Epub 2020 Jul 30.

Abstract

Ethylene glycol and glycolic acid are bulk chemicals with a broad range of applications. The ethylene glycol and glycolic acid biosynthesis pathways have been produced by microorganisms and used as a biological route for their production. Unlike the methods that use xylose or glucose as carbon sources, xylonic acid was used as a carbon source to produce ethylene glycol and glycolic acid in this study. Amounts of 4.2 g/L of ethylene glycol and 0.7 g/L of glycolic acid were produced by a wild-type Escherichia coli W3110 within 10 H of cultivation with a substrate conversion ratio of 0.5 mol/mol. Furthermore, E. coli strains that produce solely ethylene glycol or glycolic acid were constructed. 10.3 g/L of glycolic acid was produced by E. coli ΔyqhD+aldA, and the achieved conversion ratio was 0.56 mol/mol. Similarly, the E. coli ΔaldA+yqhD produced 8.0 g/L of ethylene glycol with a conversion ratio of 0.71 mol/mol. Ethylene glycol and glycolic acid production by E. coli on xylonic acid as a carbon source provides new information on the biosynthesis pathway of these products and opens a novel way of biomass utilization.

摘要

乙二醇和羟基乙酸是具有广泛应用的大宗化学品。微生物已经产生了乙二醇和羟基乙酸的生物合成途径,并将其用作生产这些物质的生物途径。与使用木糖或葡萄糖作为碳源的方法不同,在本研究中,使用戊二酸作为碳源来生产乙二醇和羟基乙酸。野生型大肠杆菌 W3110 在培养 10 小时内产生了 4.2 g/L 的乙二醇和 0.7 g/L 的羟基乙酸,底物转化率为 0.5 mol/mol。此外,还构建了仅生产乙二醇或羟基乙酸的大肠杆菌菌株。大肠杆菌 ΔyqhD+aldA 产生了 10.3 g/L 的羟基乙酸,转化率为 0.56 mol/mol。同样,大肠杆菌 ΔaldA+yqhD 产生了 8.0 g/L 的乙二醇,转化率为 0.71 mol/mol。大肠杆菌以戊二酸为碳源生产乙二醇和羟基乙酸,为这些产物的生物合成途径提供了新的信息,并为生物质利用开辟了新途径。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验