利用乙二醇氧化微生物生产乙醇酸。
Glycolic acid production using ethylene glycol-oxidizing microorganisms.
作者信息
Kataoka M, Sasaki M, Hidalgo A R, Nakano M, Shimizu S
机构信息
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
出版信息
Biosci Biotechnol Biochem. 2001 Oct;65(10):2265-70. doi: 10.1271/bbb.65.2265.
Screening for microorganisms oxidizing ethylene glycol to glycolic acid was carried out. Among stock cultures, several yeasts and acetic acid bacteria showed high glycolic acid producing activity. Pichia naganishii AKU 4267 formed the highest concentration of glycolic acid, 35.3 g/l, from 10% (v/v) ethylene glycol (molar conversion yield, 26.0%). Among soil isolates, Rhodotorula sp. 3Pr-126, isolated using propylene glycol as a sole carbon source, formed the highest concentration of glycolic acid, 25.1 g/l, from 10% (v/v) ethylene glycol (molar conversion yield, 18.5%). Rhodotorula sp. 3Pr-126 showed higher activity toward 20% (v/v) ethylene glycol than P. naganishii AKU 4267. Optimization of the conditions for glycolic acid production was investigated using P. naganishii AKU 4267 and Rhodotorula sp. 3Pr-126. Under the optimized conditions, P. naganishii AKU 4267 and Rhodotorula sp. 3Pr-126 formed 105 and 110 g/l of glycolic acid (corrected molar conversion yields, 88.0 and 92.2%) during 120 h of reaction, respectively.
开展了对将乙二醇氧化为乙醇酸的微生物的筛选工作。在保藏培养物中，几种酵母和醋酸菌表现出较高的乙醇酸生产活性。长谷西毕赤酵母AKU 4267能从10%（v/v）的乙二醇中形成最高浓度的乙醇酸，为35.3 g/l（摩尔转化率为26.0%）。在从土壤中分离得到的菌株中，以丙二醇作为唯一碳源分离得到的红酵母3Pr - 126能从10%（v/v）的乙二醇中形成最高浓度的乙醇酸，为25.1 g/l（摩尔转化率为18.5%）。红酵母3Pr - 126对20%（v/v）乙二醇的活性高于长谷西毕赤酵母AKU 4267。使用长谷西毕赤酵母AKU 4267和红酵母3Pr - 126对乙醇酸生产条件进行了优化研究。在优化条件下，长谷西毕赤酵母AKU 4267和红酵母3Pr - 126在120小时的反应过程中分别形成了105 g/l和110 g/l的乙醇酸（校正摩尔转化率分别为88.0%和92.2%）。
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