Solutions for Natural Resources and Environment, VTT Technical Research Centre of Finland Ltd, Tietotie 2, P.O. Box 1000, 02044 VTT, Espoo, Finland.
Appl Microbiol Biotechnol. 2019 Mar;103(6):2525-2535. doi: 10.1007/s00253-019-09640-2. Epub 2019 Feb 1.
Glycolic acid (GA) and ethylene glycol (EG) are versatile two-carbon organic chemicals used in multiple daily applications. GA and EG are currently produced by chemical synthesis, but their biotechnological production from renewable resources has received a substantial interest. Several different metabolic pathways by using genetically modified microorganisms, such as Escherichia coli, Corynebacterium glutamicum and yeast have been established for their production. As a result, the yield of GA and EG produced from sugars has been significantly improved. Here, we describe the recent advancement in metabolic engineering efforts focusing on metabolic pathways and engineering strategies used for GA and EG production.
乙醇酸（GA）和乙二醇（EG）是两种用途广泛的二碳有机化学品，广泛应用于日常生活中。GA 和 EG 目前是通过化学合成生产的，但利用可再生资源进行生物技术生产已经引起了人们的极大兴趣。已经建立了几种不同的代谢途径，通过使用基因修饰的微生物，如大肠杆菌、谷氨酸棒状杆菌和酵母，来生产 GA 和 EG。因此，糖生产的 GA 和 EG 的产量得到了显著提高。在这里，我们描述了代谢工程努力的最新进展，重点介绍了用于 GA 和 EG 生产的代谢途径和工程策略。
